Overview

The fourth edition provides new and updated chapters that focus on experimental models, useful for investigating various aspects of cytoskeleton structure and function.  Chapters detail protocols on animal models, plant models, protist models, fungal models, live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, genetics, and proteomics. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Cytoskeleton: Methods and Protocols, Fourth Edition aims to provide an updated version to help researchers expand their understanding of cytoskeleton structure and function.

Full Product Details

Author:   Ray H. Gavin
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   4th ed. 2022
Volume:   2364
Weight:   0.839kg
ISBN:  

9781071616635

ISBN 10:   1071616633
Pages:   430
Publication Date:   25 September 2021
Audience:   College/higher education ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

PART I                        IMAGING THE CYTOSKELETON 1          Super Resolution Imaging of the Actin Cytoskeleton in Living Cells Using TIRF-SIM             Torsten Wöllert and George M. Langford 2          Imaging Cytoskeleton Components by Electron Microscopy Tatyana Svitkina 3          Imaging the Actin Cytoskeleton in Live Budding Yeast Cells Cierra N. Sing, Emily J. Yang, Theresa C. Swayne, Ryo Higuchi-Sanabria, Catherine A. Tsang, Istvan R. Boldogh, and Liza A. Pon 4          Imaging the Actin Cytoskeleton in Fixed Budding Yeast Cells Cierra N. Sing, Emily J. Yang, Ryo Higuchi-Sanabria, Liza A. Pon, Istvan R. Boldogh, and Theresa C. Swayne 5          Imaging of actin cytoskeletal integrity during aging in C. elegans Gilberto Garcia, Stefan Homentcovschi, Naame Kelet, and Ryo Higuchi-Sanabria 6          Imaging the Cytoskeleton in Living Plant Roots Chenglin Chai, Sabrina Chin, and Elison B. Blancaflor 7          Imaging of Actin Cytoskeleton in the Nematode Caenorhabditis elegans Shoichiro Ono 8          Imaging of the Cytoskeleton using Live and Fixed Drosophila Tissue Culture Cells Derek A. Applewhite, Christine A. Lacy, Eric R. Griffis, and Omar A. Quintero     PART II                      CYTOSKELETON DYNAMICS 9          Influencing the Actin-Dynamics in Plant Cells by Jasplakinolide, Chondramides, Phalloidin, Cytochalasins, and Latrunculins Andreas Holzinger 10        Analysis of Morphogenesis and Flagellar Assembly During Spermatogenesis in Planarian Flatworms Labib Rouhana, Tracy Chong, and Phillip A. Newmark 11        ­An In Vitro Model System to Test Mechano-Microbiological Interactions Between Bacteria and Host Cells             Luis Carlos Santos, Emilia Laura Munteanu, and Nicolas Biais 12        Purification of Cytoskeletal Proteins by Fast Protein Liquid Chromatography (FPLC) Using an ÄKTA Start System Michael Gotesman, Yueyang Wang, Sharath Madasu, and Carter A. Mitchell 13        The Cilioprotist Cytoskeleton, a Model for Understanding How Cell Architecture and Pattern Are Specified: Recent Discoveries from Ciliates and Comparable Model Systems             Linda A. Hufnagel PART III                    CELL AND ORGANELLE MOTILITY 14        3D and 4D Tumorigenesis Model for the Quantitative Analysis of Cancer Cell Behavior and Screening for Anti-Cancer Drugs Deborah Wessels, Daniel F. Lusche, Edward Voss, and David R. Soll 15        Melanosome Motility in Fish Retinal Pigment Epithelial (RPE) Cells Christina King-Smith   16        Chemotaxis: Under Agarose Assay Derrick Brazill and David A. Knecht PART IV                     GENETIC AND PROTEOMIC PROTOCOLS             17        Functional Analysis of Actin-Binding Proteins in the Central Nervous System of Drosophila Qi He and Christopher Roblodowski 18        Using a Hand-Held Gene Gun for Genetic Transformation of Tetrahymena thermophila Michael Gotesman, Roland E. Hosein, and Selwyn A. Williams 19        Proteomic Tools for Analysis of Cytoskeleton Proteins Carlos Barreto, Andriele Silva, Eliza Wiech , Antonio Lopez, Avdar San, and Shaneen Singh

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