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Author:   Frances R. Balkwill (Principal Scientist, Biological Therapies Laboratory, Imperial Cancer Research Fund, London)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Edition:   2nd Revised edition
Volume:   No.155
Dimensions:   Width: 15.00cm , Height: 2.80cm , Length: 23.00cm
Weight:   0.890kg
ISBN:  

9780199635672

ISBN 10:   0199635676
Pages:   450
Publication Date:   01 September 1995
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   To order  
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Table of Contents

1: Cloning and expression of cytokine genes. 2: Southern blotting of cytokine genes. 3: Northern analysis, ribonuclease protection and in situ analysis of cytokine messenger RNA. 4: Polymerase chain reaction to quantitate cytokine mRNA. 5: Comparative polymerase chain reaction. 6: Receptor binding studies. 7: Measurement of cytokine induction of PC-specific phospholipase C and sphingomyelinases. 8: Two dimensional gel elctrophoresis for purification, sequencing and identification of cytokine-induced proteins in normal and malignant cells. 9: Antiviral activity of cytokines. 10: Assays for human B cell responses to cytokines. 11: T cells and cytokines. 12: The generation and quantitation of cell-mediated toxicity. 13: Assays for chemotaxis. 14: Cytokine regulation of endothelial cells. 15: Assays for haematopoietic growth factors. 16: Assays for macrophage activation by cytokines. 17: Measurement of proliferative, cytolytic and cytostatic activity of cytokines. 18: Production of cytokine transgenic and knockout mice. 19: Development of antibodies to cytokines. 20: Visualizing the production of cytokines and their receptors by single human cells. 21: Quantitative biological assays for individual cytokines. 22: RIA, IRMA and ELISA assays for cytokines and their soluble receptors

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