Culture of Human Embryonic Stem Cells: Methods and Techniques
Author:   Harry Moore
Publisher:   Humana Press Inc.
Edition:   2012
ISBN:  

9781603275392


Pages:   250
Publication Date:   30 November 2011
Format:   Paperback
Availability:   Awaiting stock   Availability explained
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Culture of Human Embryonic Stem Cells: Methods and Techniques


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Author:   Harry Moore
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2012
ISBN:  

9781603275392


ISBN 10:   1603275398
Pages:   250
Publication Date:   30 November 2011
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Awaiting stock   Availability explained
The supplier is currently out of stock of this item. It will be ordered for you and placed on backorder. Once it does come back in stock, we will ship it out for you.

Table of Contents

Background to embryonic stem cells.- Regulatory and ethical issues.- Setting up a lab.- Establishing a hES research programme.- Embryonic stem cell derivation.- Principles of hES cell culture.- Cryopreservation of cell lines.- Feeder-free techniques.- Advanced culture techniques for hES cells.- Avoiding culture pitfalls.- Methods of differentiation.- Differentiation Protocols.- Analysis of differentiation.- Immunofluorescent labeling for surface antigens and FACS analysis.- Flow Cytometry.- Transfection.- Molecular profiling of hES cells.- Quality control and assurance.

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