Overview

Crystallography is the major method of determining structures of biological macromolecules, yet crystallization techniques are still regarded as difficult to perform. This text continues in the vein of the first edition by providing a detailed and rational guide to producing crystals of proteins and nucleic acids of sufficient quantity and quality for diffraction studies. It has been thoroughly updated to include all the major new techniques such as the uses of molecular biology in structural biology (maximizing expression systems, sequence modifications to enable crystallization, and the introduction of anomalous scatterers); diagnostic analysis of prenucleation and nucleation by spectroscopic methods; and the two-dimensional electron crystallography of soluble proteins on planar lipid films.

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9786610756384

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