Crop Genome Editing Using CRISPR/Cas9: Theory and Practice
Author:   Ramalingam Ravindhran (Professor and Head, Department of Plant Biology and Biotechnology, Loyola College, Chennai, India) ,  Govindan Ganesan (Research Assistant Professor, Department of Genetic Engineering, SRM University, Kattankulathur, India) ,  A. Vinoth (Assistant Professor, Department of Botany, St. Xavier's College, Tamil Nadu, India)
Publisher:   Elsevier Science Publishing Co Inc
ISBN:  

9780128166727


Pages:   250
Publication Date:   01 December 2019
Format:   Paperback
Availability:   Not yet available   Availability explained
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Crop Genome Editing Using CRISPR/Cas9: Theory and Practice


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Overview

Crop Genome Editing Using CRISPR/Cas9: Theory and Practice is a highly useful reference for implementing genome engineering technologies, particularly CRISPR related projects in agricultural crops and other plants. This book provides an introduction to CRISPR's basic science and applied aspects, along with detailed protocols. It presents a detailed workflow, beginning with genome sequence retrieval and then mutation analysis in genome edited events using sequencing tools. The book helps those in the field methodically plan, design and conduct experiments. This practical guide will dramatically help researchers in accelerating conventional plant breeding programs.

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Author:   Ramalingam Ravindhran (Professor and Head, Department of Plant Biology and Biotechnology, Loyola College, Chennai, India) ,  Govindan Ganesan (Research Assistant Professor, Department of Genetic Engineering, SRM University, Kattankulathur, India) ,  A. Vinoth (Assistant Professor, Department of Botany, St. Xavier's College, Tamil Nadu, India)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
ISBN:  

9780128166727


ISBN 10:   012816672
Pages:   250
Publication Date:   01 December 2019
Audience:   College/higher education ,  Postgraduate, Research & Scholarly ,  Tertiary & Higher Education
Format:   Paperback
Publisher's Status:   Active
Availability:   Not yet available   Availability explained
This item is yet to be released. You can pre-order this item and we will dispatch it to you upon its release.

Table of Contents

1. Introduction to genome editing 2. Crop improvement using CRISPR/Cas9 3. Designing of sgRNA 4. Tools and Resources for the construction of CRISPR vectors 5. Cloning of CRISPR constructs 6. Protoplast Isolation from plants 7. Delivery of CRISPR constructs into protoplasts and mutation analysis 8. Agrobacterium mediated transformation of CRISPR constructs for the generation of stable mutant plants 9. Recent Advancements in CRISPR/Cas9 genome editing in plants

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Professor and Head, Department of Plant Biology and Biotechnology, Loyola College, Chennai, India Research Assistant Professor, Department of Genetic Engineering, SRM University, Kattankulathur, India Assistant Professor, Department of Botany, St. Xavier's College, Tamil Nadu, India

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