Overview

This volume details different laboratory techniques used to develop regenerative therapies that help treat corneal blindness. Chapters guide readers through methods and protocols on optimal cell culture conditions, gene-editing techniques, multiple types of scaffold for corneal regeneration, how to evaluate the success of these therapies, and cell and material characterization techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Corneal Regeneration: Methods and Protocols aims to be useful for new and experienced laboratory researchers working on different aspects of corneal regeneration.

Full Product Details

Author:   Mark Ahearne
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2020
Volume:   2145
Weight:   0.507kg
ISBN:  

9781071606018

ISBN 10:   1071606018
Pages:   250
Publication Date:   16 June 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Isolation and Culture of Corneal Stromal Stem Cells.- In vitro Expansion of Corneal Endothelial Cells for Transplantation.- Primary Culture of Cornea-Limbal Epithelial Cells In Vitro.- Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant.- A Guide to the Development of Human Cornea Organoids from Induced Pluripotent Stem Cells in Culture.- Gene Editing for Corneal Stromal Regeneration.- Preparation and Administration of Adeno-associated Virus Vectors for Corneal Gene Delivery.-The Self-assembly Approach as a Tool for the Tissue Engineering of a Bi-lamellar Human Cornea.- Formation of Corneal Stromal-like Assemblies using Human Corneal Fibroblasts and Macromolecular Crowding.- Preparation of Dried Amniotic Membrane for Corneal Repair.- Fabrication of Corneal Extracellular Matrix Derived Hydrogels.- Synthesis and Application of Collagens for Assembling a Corneal Implant.- Development and Validation of a 3D in vitro Model to Study the Chemotactic Behaviour of Corneal Stromal Fibroblasts.- Femtosecond Laser Assisted Surgery for Implantation of Bioengineered Corneal Stroma to Promote Corneal Regeneration.- The use of Animal Models to Assess Engineered Corneal Tissue.- X-ray Diffraction Imaging of Corneal Ultrastructure.

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