Overview

Full Product Details

Author:   Roberto Bruzzone ,  Christian Giaume
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2001 ed.
Volume:   154
Dimensions:   Width: 15.20cm , Height: 3.00cm , Length: 22.90cm
Weight:   0.881kg
ISBN:  

9780896036581

ISBN 10:   0896036588
Pages:   491
Publication Date:   01 December 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Tools to Study Connexins.- Investigation of Connexin Gene Expression Patterns by In Situ Hybridization Techniques.- Sodium Dodecyl Sulfate-Freeze-Fracture Immunolabeling of Gap Junctions.- Purification of Gap Junctions.- Culturing of Mammalian Cells Expressing Recombinant Connexins and Two-Dimensional Crystallization of the Isolated Gap Junctions.- Connexins/Connexons.- Biochemical Analysis of Connexon Assembly.- Expression and Imaging of Connexin-GFP Chimeras in Live Mammalian Cells.- Analysis of Connexin Expression in Brain Slices by Single-Cell Reverse Transcriptase Polymerase Chain Reaction.- Use of Retroviruses to Express Connexins.- Spatiotemporal Depletion of Connexins Using Antisense Oligonucleotides.- Transfection and Expression of Exogenous Connexins in Mammalian Cells.- Assays For Function.- Assaying the Molecular Permeability of Connexin Channels.- Applying the Xenopus Oocyte Expression System to the Analysis of Gap Junction Proteins.- Mutagenesis to Study Channel Structure.- Dual Patch Clamp.- Determining Ionic Permeabilities of Gap Junction Channels.- Fluorescence Recovery After Photobleaching.- Capture of Transjunctional Metabolites.- Physiology and Biology of Connexins.- The Study of Connexin Hemichannels (Connexons) in Xenopus Oocytes.- Exploring Hemichannel Permeability In Vitro.- Inducing De Novo Formation of Gap Juntion Channels.- Recording and Analysis of Putative Direct Electrical Interactions in the Mammalian Brain.- Intercellular Calcium Signaling and Flash Photolysis of Caged Compounds.- Biochemical Analysis of Connexin Phosphorylation.- How to Close a Gap Junction Channel.

Reviews

This book will be invaluable to those in the connexin field and a fascinating read and useful text to those new to this area of research. -Molecular Biotechnology

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions