Overview

This volume provides a wide range of imaging protocols that can be tailored to specific organisms or cell-types.  Chapters guide readers through fixed-cell, live-cell, phenotype screening, super-resolution, intravital imaging techniques, and fluorescence life-time imaging microscopy (FLIM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Confocal Microscopy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Full Product Details

Author:   Joseph Brzostowski ,  Haewon Sohn
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2304
Weight:   0.910kg
ISBN:  

9781071614013

ISBN 10:   1071614010
Pages:   363
Publication Date:   25 May 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Advances in Confocal Microscopy and Selected Applications.- Choosing Fluorescent Probes and Labeling Systems.- General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy.- Microfabricated devices for confocal microscopy on biological samples.- Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging.- High-resolution multicolor imaging of mitochondria in lymphocytes.- Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy.- Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy.- Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight.- Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopy in the Model Organism Dictyostelium discoideum.- Imaging GPCR-mediated Signal Events Leading to Chemotaxis and Phagocytosis.- High-throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains.- Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy.- Studying Neuronal Biology Using Spinning Disc Confocal Microscopy.- Method for Acute Intravital Imaging of the Large Intestine in Live Mice.- Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism.- Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry.- FLIM Imaging for Metabolic Studies in Live Cells.- A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM).

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