Overview

This detailed volume compiles state-of-the-art protocols that will serve as recipes for scientists researching collagen, an abundant protein with great importance to health and disease, as well as in applications like food, cosmetics, pharmaceuticals, cosmetic surgery, artificial skin, and glue. Beginning with a section on in vitro models for the characterization of collagen formation, the book continues by highlighting large-scale analysis of collagen with mass spectrometry in order to elucidate the proteomics, degradomics, interactomes, and cross-linking of collagen, high resolution imaging approaches for collagen by the use of scanning electron microscopy and multiphoton imaging, as well as the role of collagen during physiological and pathological conditions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Collagen: Methods and Protocols is an ideal guide to high quality and repeatable protocols in this vital field of study.

Full Product Details

Author:   Irit Sagi ,  Nikolaos A. Afratis
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1944
Weight:   0.737kg
ISBN:  

9781493990948

ISBN 10:   1493990942
Pages:   254
Publication Date:   07 March 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: In Vitro Models for Collagen Characterization 1. Cloning and Mutagenesis Strategies for Large Collagens Olivier Bornert and Alexander Nystroem 2. Multi-Well Fibrillation Assay as a Tool for Analyzing Crosslinking and Stabilization of Collagen Venkat Raghavan Krishnaswamy 3. Production and Characterization of Chemically Cross-Linked Collagen Scaffolds Ignacio Sallent, Hector Capella-Monsonis, and Dimitrios I. Zeugolis 4. A Biomimetic Model for Mineralization of Type-I Collagen Fibrils Shasha Yao, Yifei Xu, Changyu Shao, Fabio Nudelman, Nico A.J.M. Sommerdijk, and Ruikang Tang Part II: Proteomics Analysis of Collagen 5. Investigation of Triple-Helix Collagen Hydroxylation by Solid-State NMR Spectroscopy Wing Ying Chow 6. Measurement of Collagen Crosslinks from Tissue Samples by Mass Spectrometry Amar Joshi, Amna Zahoor, and Alberto Buson 7. Mass Spectrometry-Based Proteomics to Define Intracellular Collagen Interactomes Ngoc-Duc Doan, Andrew S. DiChiara, Amanda M. Del Rosario, Richard P. Schiavoni, and Matthew D. Shoulders 8. Exploring Extracellular Matrix Degradomes by TMT-TAILS N-Terminomics Elizabeta Madzharova, Fabio Sabino, and Ulrich auf dem Keller Part III: Imaging Approaches for Collagen 9. Sample Preparation of Extracellular Matrix of Murine Colons for Scanning Electron Microscopy Elee Shimshoni and Irit Sagi 10. In Situ Detection of Degraded and Denatured Collagen via Triple Helical Hybridization: New Tool in Histopathology Yang Li and S. Michael Yu 11. Combination of Traction Assays and Multiphoton Imaging to Quantify Skin Biomechanics Stephane Bancelin, Barbara Lynch, Christelle Bonod-Bidaud, Petr Dokladal, Florence Ruggiero, Jean-Marc Allain, and Marie-Claire Schanne-Klein 12. Analysis of Collagen-Binding Integrins Interactions with Supramolecular Aggregates of the Extracellular Matrix Uwe Hansen Part IV: Collagen in Health and Disease 13. Assessing Collagen Deposition during Aging in Mammalian Tissue and in Caenorhabditis elegans Alina C. Teuscher, Cyril Statzer, Sophia Pantasis, Mattia R. Bordoli, and Collin Y. Ewald 14. Procollagen C-Proteinase Enhancer 1 (PCPE-1) in Liver Fibrosis Efrat Kessler and Eyal Hassoun 15. Tumorigenic Interplay between Macrophages and Collagenous Matrix in the Tumor Microenvironment Chen Varol 16. FACS Analysis of Col1 Protein Levels in Primary Fibroblasts Noam Cohen and Neta Erez 17. Methods for the Construction of Collagen-Based Triple-Helical Peptides Designed as Matrix Metalloproteinase Inhibitors Gregg B. Fields

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