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OverviewA novel bacterium was engineered with an effective expression system to improve its ability to degrade oil in contaminated environments. Enrichment culture techniques were used with crude oil as the sole carbon source to isolate microorganisms. Seventeen bacterial isolates were identified using biochemical tests and 16S rRNA gene sequencing. Identified genera included Acinetobacter, Pseudomonas, Aeromonas, Micrococcus, Achromobacter, Enterobacter, Klebsiella, Staphylococcus, Bacillus, Exiguobacterium, Brevibacillus, and Stenotrophomonas. A specific primer was designed to amplify the alkB gene, confirming its presence in Pseudomonas aeruginosa. Plasmid curing confirmed the gene's presence on the chromosome. The alkB gene was cloned into a plasmid vector, transformed into E. coli, and confirmed via PCR. Expression of the gene was verified, and its contribution to biodegradation efficiency was tested using n-hexadecane as a substrate. Biodegradation efficiency increased from 32.63% to 77.42% after 72 hours, indicating a 44.8% improvement. Gene amplification and cloning strategies were simulated using SnapGene software. This study is the first of its kind in Iraq. Full Product DetailsAuthor: Fadhil N Alkanany , Rasha M OthmanPublisher: LAP Lambert Academic Publishing Imprint: LAP Lambert Academic Publishing Dimensions: Width: 15.20cm , Height: 0.90cm , Length: 22.90cm Weight: 0.222kg ISBN: 9786207467365ISBN 10: 6207467361 Pages: 144 Publication Date: 26 March 2024 Audience: General/trade , General Format: Paperback Publisher's Status: Active Availability: In Print  This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us. Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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