Overview

This updated volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and limited need for specialized reagents. These techniques are essential in laboratory fields including endocrinology, biochemical genetics, drug analysis, proteomics, and pathogen identification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Cutting-edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols, Second Edition is an ideal resource for clinical laboratory scientists who are already using or thinking of bringing mass spectrometry to their laboratories.

Full Product Details

Author:   Uttam Garg
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2546
Weight:   1.252kg
ISBN:  

9781071625644

ISBN 10:   1071625640
Pages:   547
Publication Date:   21 September 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Introduction to Mass Spectrometry for Bimolecular Analysis in a Clinical Laboratory             Y. Victoria Zhang and Uttam Garg   2. System Performance Monitoring in Clinical Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)             Shannon Haymond   3. Tandem Mass Spectrometry for the Analysis of Plasma/Serum Acylcarnitines for the Diagnosis of Certain Organic Acidurias and Fatty Acid Oxidation Disorders             David Scott, C. Clinton Frazee III, Bryce Heese, and Uttam Garg   4. Quantification of Plasma S-Adenosylmethionine and S-Adenosylhomocysteine Using Liquid Chromatography Electrospray Tandem Mass Spectrometry             Erland Arning, Brandi Wasek, and Teodoro Bottiglieri   5. Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)             J. Grace Van Der Gugten and Daniel T. Holmes   6. Comprehensive Determination of Amino Acids for Diagnosis of Inborn Errors of Metabolism             Stephen M. Roper, Annette L. Weindel, and Dennis J. Dietzen   7. Quantification of Branched-Chain Amino Acids in Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC- MS/MS)             Hamed Piri-Moghadam, Alan Miller, Debra Pronger, Faye Vicente, Joel Charrow, Shannon Haymond, and David C. Lin   8. Quantitation of Butyrylcarnitine, Isobutyrylcarnitine, and Glutarylcarnitine in Urine Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS)             Judith A. Hobert, Stephen A. Brose, and Marzia Pasquali   9. Quantification of Free and Total Carnitine in Serum Using Liquid Chromatography Tandem Mass Spectrometry             Serena Baird, C. Clint Frazee III, and Uttam Garg   10. Sensitive and Robust LC-MS/MS Analysis of Salivary Cortisol in Negative Mode             Wayne B. Anderson, Putuma P. Gqamana, and Y. Victoria Zhang   11. Measurement of Urine Free Cortisol and Cortisone by LC-MS/MS             Julie A. Ray, Erik Kish-Trier, and Lisa M. Johnson   12. Laboratory Diagnosis of Cerebral Creatine Deficiency Syndromes by Determining Creatine and Guanidinoacetate in Plasma and Urine             Ning Liu and Qin Sun   13. Quantitation of Estradiol and Testosterone in Serum Using LC-MS/MS             Ryan C. Schofield, Daniel Kirchoff, and Dean C. Carlow   14. Quantitation of Fatty Acids in Serum/Plasma and Red Blood Cells by Gas Chromatography-Negative Chemical Ionization-Mass Spectrometry             Erik Kish-Trier and Tatiana Yuzyuk   15. Quantitation of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry             Erland Arning, Brandi Wasek, and Teodoro Bottiglieri   16. A Simple, Fast, and Reliable LC-MS/MS Method for the Measurement of Homovanillic Acid and Vanillylmandelic Acid in Urine Specimens             Vrajesh Pandya and Elizabeth L. Frank   17. Quantitation of Neuroblastoma Markers Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine by Gas Chromatography-Mass Spectrometry (GC/MS)             Melissa Beals, Bheemraj Ramoo, C. Clinton Frazee III, and Uttam Garg   18. Quantification of 5-Hydroxyindoleacetic Acid in Urine by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry             Heather A. Nelson and Elizabeth L. Frank   19. Quantitation of IgG Subclasses in Serum Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)             J. Grace Van Der Gugten, Daniel T. Holmes, and Andre Mattman   20. Quantification of Insulin Analogs by Liquid Chromatography-High Resolution Mass Spectrometry             Erica M. Fatica, Nicholas E. Larkey, and Ravinder J. Singh   21. Differentiation of Common IGF-1 Variants Using HRAM-MS COM Determination with Follow-Up MS/MS Verification             Nicholas E. Larkey, Erica M. Fatica, and Ravinder J. Singh   22. Monitoring and Identifying Insulin-Like Growth Factor 1 Variants by Liquid Chromatography-High Resolution Mass Spectrometry in a Clinical Laboratory             Ievgen Motorykin, Allison Li, and Zengru Wu   23. Quantitation of Lactate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry             Brandi Wasek and Erland Arning   24. Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry             Hsuan-Chieh (Joyce) Liao and Hsiao-Jan Chen   25. Plasma Lysosphingolipid Biomarker Measurement by Liquid Chromatography-Tandem Mass Spectrometry             Brandon B. Stauffer and Chunli Yu   26. Detection of 13C-Mannitol and Other Saccharides Using Tandem Mass Spectrometry for Evaluation of Intestinal Permeability, or Leaky Gut             Nicholas E. Larkey, Erica M. Fatica, and Ravinder J. Singh   27. LC-MS/MS Method for High Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine: Method for Analyzing Isomers without Chromatographic Separation             Mark M. Kushnir, Gordon J. Nelson, Elizabeth L. Frank, and Alan L. Rockwood   28. Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry             Erland Arning, Brandi Wasek, and Teodoro Bottiglieri   29. Screening of Organic Acidurias by Gas Chromatography Mass Spectrometry (GC-MS)             David Scott, C. Clinton Frazee III, and Uttam Garg   30. Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Using Urease and Gas Chromatography-Mass Spectrometry (GC/MS)             Stanley F. Lo, Keely Pierzchalski, Velta Young, and William J. Rhead   31. Quantitative Organic Acids in Urine by Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry (GCxGC-TOFMS)             Erland Arning, Paula Ashcraft, Jeanna Bennett-Firmin, and Lawrence Sweetman   32. Quantification of Parathyroid Hormone and Its Fragments in Serum by Liquid Chromatography-High Resolution Mass Spectrometry             Erica M. Fatica, Nicholas E. Larkey, and Ravinder J. Singh   33. High Sensitivity Measurement of Parathyroid Hormone Related Protein (PTHrP) in Plasma by LC-MS/MS             Mark M. Kushnir and Alan L. Rockwood   34. Quantitation of Phenylalanine in Dried Blood Spot using Liquid Chromatography Tandem Mass Spectrometry for Monitoring of Patients with Phenylketonuria (PKU)             Serena Baird, C. Clinton Frazee III, and Uttam Garg   35. An Optimized Procedure for Proteomic Analysis of Extracellular Vesicles Using In-Stage Tip Digestion and DIA LC-MS/MS: Application to Liquid Biopsy in Cancer             Rajesh K. Soni, Lyssa Dimapanat, Manpreet S. Katari, and Alex J. Rai   36. High-Throughput Plasma Proteomic Profiling              Rajesh Kumar Soni   37. Quantitation of Purine in Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry             Qin Sun   38. Quantitation of Pyrimidine in Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry             Ning Liu and Qin Sun   39. Quantitation of Renin Activity in Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)             J. Grace Van Der Gugten and Daniel T. Holmes   40. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Method for the Quantification Steroids Androstenedione, Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone             Ada Munar, C. Clinton Frazee III, and Uttam Garg   41. A User-Friendly Sample Preparation Alternative for Manual and Automated LC-MS/MS Quantification of Testosterone             Judy A. Stone, Dawn Francisco, Heather Tone, Joshua Akin, and Robert L. Fitzgerald   42. Quantitation of Thyroglobulin in Serum Using SISCAPA and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)             J. Grace Van Der Gugten, Morty Razavi, and Daniel T. Holmes   43. Quantitation of Free Thyroxine by Equilibrium Dialysis and Liquid Chromatography-Tandem Mass Spectrometry             Weijia (William) Wu, Michael J. McPhaul, and Zengru Wu   44. Quantification of Tryptophan, Indole, and Indoxyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry             Serena Baird, C. Clinton Frazee III, and Uttam Garg   45. Very Long-Chain Fatty Acids Quantification by Gas-Chromatography Mass Spectrometry             Anna I. Scott   46. Quantification of Very Long-Chain and Branched-Chain Fatty Acids in Plasma by Liquid Chromatography-Tandem Mass Spectrometry             Irene De Biase and Marzia Pasquali   47. High Throughput Analysis of 25-OH-Vitamin D2 and D3 Using Multi-Channel Liquid Chromatography-Tandem Mass Spectrometry             Putuma P. Gqamana and Y. Victoria Zhang   48. Quantification of 25-Hydroxyvitamin D2 and D3 Using Liquid Chromatography-Tandem Mass Spectrometry             Ada Munar, Clinton Frazee III, and Uttam Garg

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