Circular RNAs: Methods and Protocols

Author:   Christoph Dieterich ,  Argyris Papantonis
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2018
Volume:   1724
ISBN:  

9781493985210


Pages:   220
Publication Date:   04 June 2019
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Circular RNAs: Methods and Protocols


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Author:   Christoph Dieterich ,  Argyris Papantonis
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2018
Volume:   1724
Weight:   0.456kg
ISBN:  

9781493985210


ISBN 10:   1493985213
Pages:   220
Publication Date:   04 June 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Detection and Reconstruction of Circular RNAs from Transcriptomic Data.- Deep Computational Circular RNA Analytics from RNA-seq Data.- Genome-wide circRNA Profiling from RNA-seq Data.- Analysis of Circular RNAs using the Web Tool CircInteractome.- Characterization and Validation of Circular RNA and their Host Gene mRNA Expression using PCR.- Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization.- Single Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as.- A Highly-efficient Strategy for Overexpressing circRNAs.- Constructing GFP-based Reporter to study Back-splicing and Translation of Circular RNA.- Northern Blot Analysis of Circular RNAs.- Non-radioactive Northern Blot of circRNAs.-  Characterization of Circular RNA Concatemers.- Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery.- Synthesis and Engineering of Circular RNAs.- Preparation of Circular RNA in vitro.- Discovering circRNA-microRNA Interactions from CLIP-Seq Data.- Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays. 

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