Overview

Cilia: From Mechanisms to Disease, Part A, Volume 175 in the Methods in Cell Biology series, offers a range of techniques and protocols that can be used to study aspects of this interesting cellular organelle both in vitro and in vivo. Sections in this new release include Protocols to induce and study ciliogenesis, Flow Cytometry-Based Approach for the study of primary Cilia, Microscopic observation of human airway ciliary movement using wheat germ agglutinin, Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins, Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method, and much more. Other sections cover Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish, Visualizing Multiciliated Cells in the Zebrafish, Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas, Using Paramecium as a Model for Ciliopathies, Using organoids to study cilia, Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model, and more.

Full Product Details

Author:   Lorenzo Galluzzi (Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA) ,  Jose Manuel Bravo-San Pedro (Departamento de Fisiología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid, Spain)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Weight:   0.450kg
ISBN:  

9780443185861

ISBN 10:   0443185867
Pages:   268
Publication Date:   21 March 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Protocols to induce and study ciliogenesis Jose Manuel M. Bravo San Pedro 2. A Flow Cytometry-Based Approach for the study of primary Cilia Francesca Ciccolini 3. Microscopic observation of human airway ciliary movement using wheat germ agglutinin Yo Kishimoto 4. Time-lapse imaging of primary cilia behavior with physiological expression of fluorescent ciliary proteins Koji Ikegami 5. Evaluation of ciliary-GPCR dynamics using a validated organotypic brain slice culture method Yumiko Saito and Yuki Kobayashi 6. High-efficient CRISPR/Cas9-mediated gene targeting to establish cell models of ciliopathies Tatsuo Miyamoto, Kosuke Hosoba, Tomoka Morita, Ying Zhang, Hiroko Kishi and Takashi Yamamoto 7. Studying the morphology, composition and function of the photoreceptor primary cilium in zebrafish Ruxandra Bachman-Gagescu 8. Visualizing Multiciliated Cells in the Zebrafish Rebecca Ann Wingert 9. Isolation of Ciliary Ectosomes and Analysis of Peptide-mediated Chemotaxis in Chlamydomonas Stephen M. King 10. Using Paramecium as a Model for Ciliopathies Judith Van Houten 11. Using organoids to study cilia Vincent GUEN 12. Using in vivo cerebellar electroporation to study neuronal cell proliferation and differentiation in a Joubert syndrome mouse model Tang K. Tang, Ting-Yu Chen and Chia-Hsiang Chang

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Author Information

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Jose Manuel Bravo-San Pedro is currently a researcher at the Department of Physiology of the Complutense University of Madrid thanks to a Ramon y Cajal contract grant. He got his Ph.D. in biochemistry, cellular biology and genetics from the University of Extremadura (Caceres, Spain) in 2011, and he did a post-doctoral stage in the laboratory of Prof. Guido Kroemer. His main research interests have always been linked to autophagy, addressing this cellular process associated with neurodegenerative diseases or cancer and recently obesity and specifically related to problems in the correct functioning of the cilium. He is co-inventor of two patents and co-author of 110 publications indexed in PubMed in prestigious international journals, with h-index 45 and 23768 cites (Dec 2022).

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