Overview

This detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through maintaining the native physiological context through the investigation of living, functional cells. In addition, the chapters reflect the dramatic improvement in the length scale of precision by utilizing single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA as well as using methods that can controllably manipulate single DNA molecules, and the use of advanced computational methods and mathematical analysis is also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.

Full Product Details

Author:   Mark C. Leake
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2476
Weight:   0.845kg
ISBN:  

9781071622209

ISBN 10:   107162220
Pages:   330
Publication Date:   31 May 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. A Next Generation of Advances in Chromosome Architecture             Mark C. Leake   2. Single Molecule Narrowfield Microscopy of Protein-DNA Binding Dynamics in Glucose Signal Transduction of Live Yeast Cells             Adam J.M. Wollman and Mark C. Leake   3. Convolutional Neural Networks for Classifying Chromatin Morphology in Live Cell Imaging             Kristina Ulicna, Laure T.L. Ho, Christopher J. Soelistyo, Nathan J. Day, and Alan R. Lowe   4. Fluorescence Recovery After Photobleaching (FRAP) to Study Dynamics of the Structural Maintenance of Chromosome (SMC) Complex in Live Escherichia coli Bacteria             Anjana Badrinarayanan and Mark C. Leake   5. Atomic Force Microscopy of DNA and DNA-Protein Interactions             Philip J. Haynes, Kavit H.S. Main, and Alice L.B. Pyne   6. Live-Cell Visualization of DNA Transfer and Pilus Dynamics during Bacterial Conjugation             Kelly Goldlust, Agathe Couturier, Laurent Terradot, and Christian Lesterlin   7. Transverse Magnetic Tweezers Allowing Coincident Epi-Fluorescence Microscopy on Horizontally Extended DNA             Stephen J. Cross, Claire E. Brown, and Christoph G. Baumann   8. Atomistic Molecular Dynamics Simulations of DNA in Complex 3D Arrangements for Comparison with Lower Resolution Structural Experiments             George Watson, Victor Velasco-Berrelleza, and Agnes Noy   9. Replication Labeling Methods for Super-Resolution Imaging of Chromosome Territories and Chromatin Domains             Ezequiel Miron, Joseph Windo, Fena Ochs, and Lothar Schermelleh   10. DNA-Protein Interactions Studied Directly Using iSCAT Imaging of GNP-Tagged Proteins             Robert J. Charman and Neil M. Kad   11. Escherichia coli Chromosome Copy Number Measurement Using Flow Cytometry Analysis             Michelle Hawkins, John Atkinson, and Peter McGlynn   12. Dynamics of Bacterial Chromosomes by Locus Tracking in Fluorescence Microscopy             Leonardo Mancini, Estelle Crozat, Avelino Javer, Marco Cosentino Lagomarsino, and Pietro Cicuta   13. Measuring Nuclear Mechanics with Atomic Force Microscopy             Ália dos Santos, Florian Rehfeldt, and Christopher P. Toseland   14. Copy Number Analysis of the Yeast Histone Deacetylase Complex Component Cti6 Directly in Living Cells             Sviatlana Shashkova, Thomas Nyström, and Mark C. Leake   15. Super-Resolution Microscopy and Tracking of DNA-Binding Proteins in Bacterial Cells             Chloé J. Cassaro and Stephan Uphoff   16. Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy             Maike Steindel, Igor de Almeida, Stanley Strawbridge, Valentyna Chernova, David Holcman, Aleks Ponjavic, and Srinjan Basu   17. The End Restraint Method for Mechanically Perturbing Nucleic Acids In Silico             Jack W. Shepherd and Mark C. Leake   18. Visualizing the Replisome, Chromosome Breaks, and Replication Restart in Bacillus subtilis             Hannah Gaimster, Charles Winterhalter, Alan Koh, and Heath Murray   19. High-Throughput Imaging of Bacillus subtilis             Paula Montero Llopis, Ryan Stephansky, and Xindan Wang   20. Measuring Nuclear Organization of Proteins with STORM Imaging and Cluster Analysis             Ália dos Santos, Rosemarie E. Gough, Lin Wang, and Christopher P. Toseland   21. Single-Molecular Quantification of Flowering Control Proteins within Nuclear Condensates in Live Whole Arabidopsis Root             Alex L. Payne-Dwyer and Mark C. Leake

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