Overview

Clear, step-by-step instructions provide you with all the techniques used in both classical and molecular cytogenetics. The emphasis is on the use of human material, but the techniques are designed to be readily applicable to other animal chromosomes. Chapters feature methods used in the preparation of chromosomes, in analyzing chromosomes directly at the light-microscope level, and in molecular genetics techniques.

Full Product Details

Author:   John R. Gosden
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1994 ed.
Volume:   29
Dimensions:   Width: 15.50cm , Height: 3.00cm , Length: 23.50cm
Weight:   1.048kg
ISBN:  

9780896032897

ISBN 10:   0896032892
Pages:   509
Publication Date:   07 February 1994
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution.- Chromosome Preparation from Hematological Malignancies.- Meiotic Chromosome Preparation.- Preparation of Chromosomes for Scanning Electron Microscopy.- Immortalized Cell Lines.- Chromosome Banding and Identification Absorption Staining.- Chromosome Banding and Identification.- Chromosome Banding.- Inhibition of Chromosome Condensation.- Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity.- Automatic Karyotype Analysis.- Bivariate Chromosome Analysis Using a Commercial Flow Cytometer.- Chromosome Sorting by Flow Cytometry.- Electrophoretic Karyotype Analysis Pulsed Field Gel Electrophoresis.- Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations.- Immunocytochemical Techniques Applied to Meiotic Chromosomes.- Immortalized Cell Lines.- Oligonucleotide Primed in Situ DNA Synthesis (PRINS).- Electron Microscopic Localization of in Situ Hybrids.- Protocols for Chromosome-Mediated Gene Transfer.- Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines.- Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping.- Chromosome Dissection and Cloning.- Molecular Analysis of Chromosome Aberrations in Hematological Malignancies.- Molecular Analysis of Chromosome Aberrations.- Alu- and LI-Primed PCR-Generated Probes for Nonisotopie in Situ Hybridization.- Chromosome Substructure Investigation.

Reviews

...a very worthwhile book...invaluable for those trying to venture into the area of molecular cytogenetics...Provides a ready source of both old and new cytogenetic methods and contains a wealth of troubleshooting tips from experienced workers in the field. - Modern Pathology

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