Overview

This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 

Full Product Details

Author:   Ricardo Borges
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2565
Weight:   0.925kg
ISBN:  

9781071626702

ISBN 10:   1071626701
Pages:   373
Publication Date:   08 October 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

  Part I   Culturing and morphology   1. Rat pheochromocytoma PC12 cells in culture Chin-Tien Wang   2. Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation Alejandra Boronat-Garcia, Marcela Palomero-Rivero, Magdalena Guerra-Crespo   3. Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells Keita Harada, Hidetada Matsuoka, Masumi Inoue   4. Transmission electron microscopy: a method for studying the adrenochromaffin cells Anna Capaldo, Maria De Falco, Luigi Rosati and Vincenza Laforgia   5. Immunogold for protein location in chromaffin cells Rafael Luján, Rocio Alfaro-Ruiz and Carolina Aguado   6. Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy Chung Yu Chan, Sue Han, Xin Wang, Xiaoli Guo and Ling-Gang Wu   Part II   Stimulus-secretion coupling   7. Online detection of catecholamine release from the perfused rat adrenal gland Ricardo Borges, Ana de la Iglesia and Antonio G. García   8. On-line recording of perifused chromaffin cells Ricardo De Pascual and Luis Gandía   9. Recording of chromaffin cell electrical activity in situ in acute adrenal slices Nathali Guerineau   10. Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats Tzitzitlini Alejandre-García, Pedro Segura-Chama, Oscar Javier Parada-Parra, Diana Millán-Aldaco and Arturo Hernández-Cruz.   11. Measurements of calcium in chromaffin cell organelles using targeted aequorins. Jaime Santo-Domingo, Pilar Álvarez-Illera, Pablo Montenegro, Rosalba I Fonteriz, Mayte Montero and Javier Álvarez   12. Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements Muhmmad Omar-Hmeadi, Liangwen Liu, Santiago Echeverry, and Sebastian Barg   13. Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell Elizabeth P. Seward and Robert C. Wykes   14. Quantal release analysis of electrochemically active molecules using single-cell amperometry Natalia Domínguez, Pablo Montenegro and J. David Machado   15. Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs Giulia Tomagra, Claudio Franchino, Emilio Carbone, Andrea Marcantoni, Alberto Pasquarelli, Federico Picollo, Valentina Carabelli   16. Vesicle collision protocols for the study of quantum size and exocytotic fraction released Soodabeh Majdi, Alex S. Lima and Andrew Ewing   17. Patch Amperometry and Intracellular Patch Electrochemistry Eugene V. Mosharov and Manfred Lindau   18. Artificial cells for dissecting exocytosis Yuanmo Wang and Ann-Sofie Cans   Part III   Chromaffin granules and membrane traffic   19. Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells Arlek Gonzalez-Jamett, María Constanza Maldifassi, Ana María Cárdenas   20. Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells José Villanueva, Yolanda Gimenez-Molina and Luis M. Gutiérrez   21. Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging Adekunle T. Bademosi and Frédéric A. Meunier     Part IV   Miscellanea   22. Determination of catecholamines in a small volume (25µl) of plasma from conscious mouse tail vein Kechun Tang and Sushil K. Mahata   23. Quantification of chromogranin A and its fragments in biological fluids   Flavio Curnis, Barbara Colombo and Angelo Corti   24. Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds María F. Cano Abad and Manuela G. López

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