Overview

This detailed volume provides methods to guide assay development, procedures designed to investigate the chemokine and glycosaminoglycan (GAG) networks, as well as their interactions, in a wide range of organs and tissues in disease and in health. The initial chapters in this book present in vivo models used to examine the roles of chemokines and GAGs in normal physiology and in the pathophysiology of disease. The book then explores present cell- and tissue-based in vitro assays to examine chemokine:GAG interactions. Finally, analytic approaches are presented that provide assays for measuring GAGs, chemokines, and cellular responses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Chemokine-Glycosaminoglycan Interactions: Methods and Protocols serves as an ideal guide for researchers seeking to analyze chemokine and GAG functions, interactions, and molecular mechanisms in vivo and in vitro.

Full Product Details

Author:   Alexandra R. Lucas
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2597
Weight:   0.729kg
ISBN:  

9781071628348

ISBN 10:   1071628348
Pages:   262
Publication Date:   15 November 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy             Laura Kutz, Tong Zhou, Qi Chen, and Hua Zhu   2. Remote Ischemic Pre-Conditioning (RIPC)             Kyung Eun Lee, Jongsoo Kim, Ki Ho Park, Jianjie Ma, and Hua Zhu   3. Mouse Corneal Transplantation             Peng Chen, Ki Ho Park, Liqiang Zhang, Alexandra R. Lucas, Heather L. Chandler, and Hua Zhu   4. Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant             Yanlin Ma and Hao Chen   5. Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine:GAG Interaction and Therapeutic Blockade             Isabela R. Zanetti, Liqiang Zhang, Michelle Burgin, Jacquelyn Kilbourne, Jordan R. Yaron, David Fonseca, and Alexandra R. Lucas   6. Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion             Sanne L. Maas, Remco T.A. Megens, and Emiel P.C. van der Vorst   7. In Vitro (Trans)migration Experiment Using Chemokines as Stimulatory Factor             Sanne L. Maas and Emiel P.C. van der Vorst   8. The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins             Wesley M. Tierney and Ian B. Hogue   9. Flow Cytometry Analysis of Immune Cell Responses             Kavita R. Manhas and Joseph N. Blattman   10. Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence             Bruno Hernaez and Antonio Alcamí   11. Protein Microarrays and Their Fabrication             Mahasish Shome and Joshua LaBaer   12. NMR Methods for Characterization of Glycosaminoglycan-Chemokine Interactions             Vitor H. Pomin and Krishna Rajarathnam   13. Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG-Protein Interactions             Amrita Basu and Ryan J. Weiss   14. Isolation and Compositional Analysis of Glycosaminoglycans             Stephanie Archer-Hartmann, Lauren E. Pepi, Christian Heiss, and Parastoo Azadi   15. How to Design Peptides             Joseph Dodd-o, Amanda M. Acevedo-Jake, Abdul-Rahman Azizogli, Vikram Khipple Mulligan, and Vivek A. Kumar   16. Methods to Assess Chemokine Binding and Anti-Chemotactic Activity of Virus Proteins             Elham Torbati, Gabriella Stuart, Kurt Krause, Chris Brown, and Lyn Wise   17. Producing Biologics with Defined N-Glycosylation in Plants             Adrian Esqueda and Qiang Chen   18. Native Deglycosylation and Size Exclusion Chromatography of Viral Chemokine Binding Proteins for Structural Discovery             Ryan Boyd, Liqiang Zhang, and Petra Fromme

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