Cervical Cancer: Methods and Protocols

Author:   Daniel Keppler ,  Athena W. Lin
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2015
Volume:   1249
ISBN:  

9781493952854


Pages:   413
Publication Date:   22 September 2016
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Cervical Cancer: Methods and Protocols


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Author:   Daniel Keppler ,  Athena W. Lin
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2015
Volume:   1249
Dimensions:   Width: 17.80cm , Height: 2.20cm , Length: 25.40cm
Weight:   7.989kg
ISBN:  

9781493952854


ISBN 10:   1493952854
Pages:   413
Publication Date:   22 September 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer.- A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for the Detection and Typing of Human Papillomavirus.- Replication of Human Papillomavirus in Culture.- HPV Binding Assay to Laminin-332/Integrin α6β4 on Human Keratinocytes.- Methods to Assess the Nucleocytoplasmic Shuttling of the HPV E1 Helicase and Its Effects on Cellular Proliferation and Induction of a DNA Damage Response.- Genetic Methods for Studying the Role of Viral Oncogenes in the HPV Life Cycle.- Robust HPV-18 Production in Organotypic Cultures of Primary Human Keratinocytes.- A High-Throughput Cellular Assay to Quantify the p53-Degradation Activity of E6 from Different Human Papillomavirus Types.- Retroviral Expression of Human Cystatin Genes in HeLa Cells.- Molecular Analysis of Human Papillomavirus Virus-Like Particle Activated Langerhans Cells In Vitro.- Selective Silencing of Gene Target Expression By siRNA Expression Plasmids in Human Cervical Cancer Cells.- Silencing of E6/E7 Expression in Cervical Cancer Stem-Like Cells.- Two-Step Procedure for Evaluating Experimentally Induced DNA Damage: Texas Red and Comet Assays.- Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe.- Immunocytochemical Analysis of the Cervical Pap Smear.- Diagnosis of HPV-Negative, Gastric-Type Adenocarcinoma of the Endocervix.- Targeting of the HPV-16 E7 Protein by RNA Aptamers.- The Use of MYBL2 as a Novel Candidate Biomarker of Cervical Cancer.- Fixation Methods for the Preservation of Morphology, RNAs, and Proteins in Paraffin-Embedded Human Cervical Cancer Cell Xenografts in Mice.- Assessment of the HPV DNA Methylation Status in Cervical Lesions.- MeDIP-on-Chip for Methylation Profiling.- Use of DBD-FISH for the Study of Cervical Cancer Progression.- A Quantitative and High-Throughput Assay of Human Papillomavirus DNAReplication.- Native Human Papillomavirus Production, Quantification, and Infectivity Analysis.- Functional Analysis of HPV-Like Particle-Activated Langerhans Cells In Vitro.- Assessment of the Radiation Sensitivity of Cervical Cancer Cell Lines.- Mouse Model of Cervicovaginal Papillomavirus Infection.- Establishment of Orthotopic Primary Cervix Cancer Xenografts.- Generation of K14-E7/∆N87ßcat Double Transgenic Mice as a Model of Cervical Cancer.

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