Cellular Quiescence: Methods and Protocols

Author:   H. Daniel Lacorazza
Publisher:   Humana Press Inc.
Edition:   1st ed. 2018
Volume:   1686
ISBN:  

9781493973705


Pages:   303
Publication Date:   18 October 2017
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Cellular Quiescence: Methods and Protocols


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Author:   H. Daniel Lacorazza
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2018
Volume:   1686
Weight:   7.392kg
ISBN:  

9781493973705


ISBN 10:   1493973703
Pages:   303
Publication Date:   18 October 2017
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Molecular Regulation of Cellular Quiescence: A Perspective from Adult Stem Cells and Its Niches.- An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts.- Flow Cytometric Detection of G0 in Live Cells by Hoechst 33342 and Pyronin Y Staining.- Using Carboxy Fluorescein Succinimidyl Ester (CFSE) to Identify Quiescent Glioblastoma Stem-Like Cells.- Isolation of Neural Stem and Progenitor Cells from the Adult Brain and Live Imaging of Their Cell Cycle with the FUCCI System.- Determination of Histone 2B-Green Fluorescent Protein (GFP) Retention in Intestinal Stem Cells.- Detecting Hematopoietic Stem Cell Proliferation Using BrdU Incorporation.- Cell Cycle Analysis by Mass Cytometry.- Preparation and Analysis of Saccharomyces cerevisiae Quiescent Cells.- Identifying Quiescent Stem Cells in Hair Follicles.- Single EDL Myofiber Isolation for Analyses of Quiescent and Activated Muscle Stem Cells.- Investigating Cellular Quiescence of T Lymphocytes and Antigen-Induced Exit from Quiescence.- Retroviral Transduction of Quiescent Murine Hematopoietic Stem Cells.- Analysis of Murine Hematopoietic Stem Cell Proliferation During Inflammation.- A Facile, In Vitro 384-Well Plate System to Model Disseminated Tumor Cells in the Bone Marrow Microenvironment.- Distinguishing States of Arrest: Genome-Wide Descriptions of Cellular Quiescence Using ChIP-Seq and RNA-Seq Analysis.- Analysis of lncRNA-Protein Interactions by RNA-Protein Pull-Down Assay and RNA Immunoprecipitation (RIP).- Analysis of MicroRNA-Mediated Translation Activation of In Vitro Transcribed Reporters in Quiescent Cells.- Genome-Wide Identification of Transcription Factor Binding Sites in Quiescent Adult Neural Stem Cells.- Study Quiescence Heterogeneity by Coupling Single-Cell Measurements and Computer Modeling.

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