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Author:   Aleksandra Skirycz ,  Marcin Luzarowski ,  Jennifer C. Ewald
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2554
Weight:   0.522kg
ISBN:  

9781071626269

ISBN 10:   1071626264
Pages:   260
Publication Date:   02 October 2023
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Protein-Metabolite Interactions Shape Cellular Metabolism and Physiology.- Affinity Purification Protocol Starting with a Small Molecule as Bait.- Cellular Thermal Shift Assay for the Detection of Small Molecule-Target Interactions in Arabidopsis Cells.- Solvent-Induced Protein Precipitation for Drug Target Discovery.- Identification of Plant Protein-Metabolite Interactions by Limited Proteolysis-Coupled Mass Spectrometry (LiP-MS).- Limited Proteolysis-Mass Spectrometry to Identify Metabolite-Protein Interactions.- Proteome Integral Solubility Alteration (PISA) for High-Throughput Ligand Target Deconvolution with Increased Statistical Significance and Reduced Sample Amount.- Parallel Analysis of Protein–Protein and Protein–Metabolite Complexes Using a Single-Step Affinity Purification.- Detecting and Characterizing Interactions of Metabolites with Proteins by Saturation Transfer Difference Nuclear Magnetic Resonance (STD-NMR) Spectroscopy.- PROMIS: Co-Fractionation Mass Spectrometry for Analysis of Protein-Metabolite Interactions.- Metabolomics: Going Deeper, Going Broader, Going Further.- Specifics of Metabolite-Protein Interactions and Their Computational Analysis and Prediction.- Biophysical Approaches for the Characterization of Protein-Metabolite Interactions.- Databases and Tools to Investigate Protein-Metabolite Interactions.

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