Overview

This updated edition explores assessing cell viability as a measure for cell fitness under conditions of physiological and patho-physiological stress as well as challenging conditions to cellular and tissue homeostasis, and accounts for the ongoing 2D-to-3D development with topics and assays that target cell viability, mobility, and functionality of tissues and organs, natural or bioartificial, in 3D. The book’s contents span a wide range of viability and functionality assays, from impedance spectroscopy to chemiluminescence, fluorescence and label-free optical detection methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Cell Viability Assays: Methods and Protocols, Second Edition serves as a valuable resource to the growing community in bioinspired life sciences, biomedical sciences, and biotechnology by providing more standardized protocols to probe the “wellbeing” of cells in various environments.

Full Product Details

Author:   Oliver Friedrich ,  Daniel F. Gilbert
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2023
Volume:   2644
Weight:   1.165kg
ISBN:  

9781071630518

ISBN 10:   1071630512
Pages:   502
Publication Date:   05 May 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Single Cell Assays in 2D Environments   1. Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness             Natalia Smolina, Aleksandr Khudiakov, and Anna Kostareva   2. The MTT Assay: A Method for Error Minimization and Interpretation in Measuring Cytotoxicity and Estimating Cell Viability             Mahshid Ghasemi, Sisi Liang, Quang Minh Luu, and Ivan Kempson   3. Assaying Proliferation Characteristics of Cells Cultured Under Static Versus Periodic Conditions             Daniel F. Gilbert, Oliver Friedrich, and Joachim Wiest   4. Network Reconstruction as a Novel High-Level Marker of Functional Neuronal Viability             Jana Katharina Dahlmanns and Marc Dahlmanns   5. Assaying Mitochondrial Function by Multi-Parametric Flow Cytometry             Hannah C. Alberico, Jonathan L. Tilly, and Dori C. Woods   6. High-Efficiency Single Cell Electrical Impedance Spectroscopy             Yongxiang Feng, Liang Huang, Peng Zhao, Fei Liang, and Wenhui Wang   7. Cell Viability Multiplexing: Quantification of Cellular Viability by Barcode Flow Cytometry and Computational Analysis             Valentina Giudice, Victoria Fonseca, Carmine Selleri, and Massimo Gadina   8. Average Rheological Quantities of Cells in Monolayers             Santanu Kumar Basu, Haider Dakhil, and Andreas Wierschem   9. Assaying Spontaneous Network Activity and Cellular Viability Using Multi-Well Microelectrode Arrays             Seline S. Choo, Jackson Y. Keever, Jasmine Brown, Jenna D. Strickland, and Timothy J. Shafer   10. Quantitative Live-Cell Ca2+ Imaging During Isotropic Cell Stretch             Ulrike Schöler, Anna-Lena Merten, Sebastian Schürmann, and Oliver Friedrich   11. Assessment of Cell Viability in Electrically Excitable Muscle Cells through Intact Twitch Stimulation             Julian Bauer, Stewart I. Head, and Oliver Friedrich   12. Evaluating Cellular Viability by iTRAQ Proteomic Profiling             Anne Poljak, Mark Raftery, and Patsie Polly   13. Quantifying pH in Malaria Using pHluorin and Flow Cytometry             Jeffrey Agyapong and Petra Rohrbach   14. Cell Membrane State, Permeability, and Elasticity Assessment for Single Cells and Cell Ensembles             Nicolas Färber, Simon V. Neidinger, and Christoph Westerhausen   15. Neutral Red Uptake Assay to Assess Cytotoxicity In Vitro             Robim M. Rodrigues, Marth Stinckens, Gamze Ates, and Tamara Vanhaecke   16. Digital Holographic Microscopy to Assess Cell Behavior             Brad Bazow, Van K. Lam, Thuc Phan, Byung Min Chung, George Nehmetallah, and Christopher B. Raub   17. Second Harmonic Generation Morphometry of Muscle Cytoarchitecture in Living Cells             Dominik Schneidereit, Stefanie Nübler, and Oliver Friedrich   18. Optimization of Cell Viability Assays for Drug Sensitivity Screens             Peter Larsson and Toshima Z. Parris   19. Cellasys #8: A Microphysiometric Assay for Real-Time Cell Analysis within 24 Hours             Sebastian Eggert, Svitlana Stetsenko, and Joachim Wiest   20. Live Cell Adhesion, Migration, and Invasion Assays             Jordi Pijuan, Anna Macià, and Anaïs Panosa   21. Cell Viability and Immunogenic Function of T Cells Loaded with Nanoparticles for Spatial Guidance in Magnetic Fields             Felix Pfister, Christoph Alexiou, and Christina Janko   Part II: Single Cell Assays in 3D Environments   22. Transwell In Vitro Cell Migration and Invasion Assays             Calvin R. Justus, Mona A. Marie, Edward J. Sanderlin, and Li V. Yang   23. Live Cell Calcium Imaging of Non-Adherent Cells             Lydia J. Bye, Rocio K. Finol-Urdaneta, and David J. Adams   24. FUCCI Reporter Gene-Based Cell Cycle Analysis             Lena Fischer and Ingo Thievessen   25. Cell Viability Assays for 3D Cellular Constructs             Zachary Congress, Matthew Brovold, and Shay Soker   26. Toxicological Analysis by Assessment of Vascularization and Cell Viability Using the Chicken's Chorioallantoic Membrane (CAM Assay)             Nadine Wiesmann, Jürgen Brieger, and Jonas Eckrich   27. Assessment of Tissue Viability by Functional Imaging of Membrane Potential             Callum M. Zgierski-Johnston and Peter Kohl   28. Multiplexed Viability Assays for High-Throughput Screening of Spheroids of Multiple Sizes             Mohana Marimuthu and Thomas Gervais   29. Tracking Gut Motility in Organ and Cultures             Peng Du, Vikram Joshi, and Arthur Beyder   30. Optimized Method of 3D Scaffold Seeding, Cell Cultivation, and Monitoring Cell Status for Bone Tissue Engineering             Adrian Krolinski, Kai Sommer, Johanna Wiesner, Oliver Friedrich, and Martin Vielreicher

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