Overview

Isolating specific cells or fractionating viable cell populations is an essential step of many of the techniques used across a wide range of biological and related disciplines. This text aims to inform those working in all areas of the biological sciences of the methods which are available to them and which is likely to be the most useful. The contributing authors have provided a comprehensive guide to the methods used to prepare cell suspensions and to separate viable cells depending on their different characteristics - such as size, density, surface charge and immunological identity. Each chapter offers direct practical guidance for the various techniques and describes the advantages and limitations of each. Reproducible protocols, hints and tips for success and troubleshooting suggestions are provided.

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9780199635795

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<br> In this reference British researchers have contributed articles covering the process of cell separation using a number of different methods, with explanations of the theoretical bases for these procedures. The chapters explain when a particular method would be best used, based on cell size, density, surface charge, hydrophobic surface properties, and antigen status. The methods described include sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immuno methods (including magnetic, column, and panning techniques), and free flow electrophoresis. --SciTech Book News<p><br> The title of this book describes the contents within, and reviews a subject that is underrepresented in the literature. The seven chapters discuss the preparation of single cell suspensions and six typical separation methods: sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immunomethods (magnetic, column, and panning techniques), and free flow electrophoresis. The descriptions emphasize practical applications of these techniques including protocols for most, if not all methods. Although the theoretical basis of these techniques are not heavily emphasized, adequate references are given for those interested. --The Quarterly Review of Biology<p><br>

In this reference British researchers have contributed articles covering the process of cell separation using a number of different methods, with explanations of the theoretical bases for these procedures. The chapters explain when a particular method would be best used, based on cell size, density, surface charge, hydrophobic surface properties, and antigen status. The methods described include sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immuno methods (including magnetic, column, and panning techniques), and free flow electrophoresis. --SciTech Book News<br> The title of this book describes the contents within, and reviews a subject that is underrepresented in the literature. The seven chapters discuss the preparation of single cell suspensions and six typical separation methods: sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immunomethods (magnetic, column, and panning techniques), and free flow electrophoresis. The descriptions emphasize practical applications of these techniques including protocols for most, if not all methods. Although the theoretical basis of these techniques are not heavily emphasized, adequate references are given for those interested. --The Quarterly Review of Biology<br>

<br> In this reference British researchers have contributed articles covering the process of cell separation using a number of different methods, with explanations of the theoretical bases for these procedures. The chapters explain when a particular method would be best used, based on cell size, density, surface charge, hydrophobic surface properties, and antigen status. The methods described include sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immuno methods (including magnetic, column, and panning techniques), and free flow electrophoresis. --SciTech Book News<br> The title of this book describes the contents within, and reviews a subject that is underrepresented in the literature. The seven chapters discuss the preparation of single cell suspensions and six typical separation methods: sedimentation, centrifugal elutriation, partitioning in aqueous two-phase systems, flow cytometry, immunomethods (magnetic, column, and panning tec

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