Overview

This third edition summarizes the most important areas of Cell-Penetrating Peptides (CPP) research. Chapters are divided into three parts detailing the historical background of CPP, the classifications of the available CPPs, approaches for prediction of novel CPPs, methods for studies of “naked” CPPs, and a brief summary of functionality issues of CPPs, both in vitro and in vivo. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Cell Penetrating Peptides: Methods and Protocols, Third Edition aims to be a useful practical guide to researches to help further their study in this field.

Full Product Details

Author:   Ülo Langel
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   3rd ed. 2022
Volume:   2383
Weight:   1.222kg
ISBN:  

9781071617540

ISBN 10:   1071617540
Pages:   632
Publication Date:   13 November 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

 PART I: Introduction, selection, mechanisms.   1. Cell-penetrating peptides Matjaž Zorko and Ülo Langel   2. Shuttling homeoproteins and their biological significance Alain Prochiantz and Ariel Di Nardo   3. Artificial evolutionary optimization process to improve the functionality of CPPs Niels Röckendorf, Katrin Ramaker, and Andreas Frey   4. In silico design of chemically modified cell penetrating peptides Vinod Kumar, Gajendra Pal Singh Raghava   5. Synthetic Molecular Evolution of Cell Penetrating Peptides William C. Wimley   6. A native CPP from rattlesnake with therapeutic and theranostic properties Lucas C. Porta, Joana D’Arc Campeiro, and Mirian A. F. Hayashi   7. Synthesis of cell-penetrating peptide coated silica nanoparticles and their physicochemical and biological characterization Isabel Gessner, Annika Klimpel and Ines Neundorf   8. Enhancing the therapeutic potential of extracellular vesicles using peptide technology Carla Martin-Perez, Mariana Conceição, Richard Raz, Matthew J. A. Wood, and Thomas C. Roberts   9. Challenges and methods for the study of CPP translocation mechanisms  Astrid Walrant, Françoise Illien, Sandrine Sagan, and Nicolas Rodriguez   10. Membrane molecular interactions and induced structures of CPPs Fatemeh Madani and Astrid Gräslund   11. A single GUV method for revealing the action of cell-penetrating peptides in biomembranes Md. Mizanur Rahman Moghal, Madhabi Lata Shuma, Md. Zahidul Islam, and Masahito Yamazaki   12. Methods for Small-Angle Scattering Measurements on Peptiplexes of DNA with Cell-Penetrating Peptides Emerson R.Silva, Lucas R. Mello, and Ian Hamley   13. Imaging CPP delivery mechanisms of oligonucleotides Najoua El Boujnouni, Alexander H. van Asbeck, Jürgen Dieker, Derick G. Wansink, and Roland Brock   14. Quantitative subcellular analysis of cyclic cell-penetrating peptide EJP18 in non-adherent cells Edward J. Sayers, Victoria L. Barlow, Yu-Hsuan Tsai, and Arwyn T. Jones   15. PepFect14: signaling and transfection Luca Falato, Maxime Gestin, and Ûlo Langel   16. Utilisation of cell-penetrating peptides for in vivo delivery of bioactive cargo – the effect of nanoparticle formulation Piret Arukuusk, Heleri Heike Härk, and Ülo Langel   Part II: Delivery of proteins and protein mimics.   17. Protein delivery by PTDs/CPPs Caroline Palm-Apergi and Steve F. Dowdy   18. Protein Delivery to Cytosol by Cell-Penetrating Peptide Bearing a Tandem Repeat Penetration-Accelerating Sequence Akiko Okuda and Shiroh Futaki   19.Recombinant transcription factors (TFs) fused to ZEBRA minimal transduction domain (MD) for modulation of mRNA transcripts. Benjamin Caulier, Jean-Luc Lenormand, and David Laurin   20. Protein mimicry and the design of bioactive cell penetrating peptides: The genesis of STOPSPERM bioportides John Howl*, Joana Vieira Silva,, Margarida Fardilha, and Sarah Jones   21. Lipopeptide pepducins as therapeutic agents Emily Michael, Lidija Covic, and Athan Kuliopulos   22. Delivery of Antibiotics by Cell-Penetrating Peptides to Kill Intracellular Pathogens Christian Rüter   23. CPP applications in immune modulation and disease therapy Ja-Hyun Koo, Won-Ju Kim, and Je-Min Choi   Part III: Targeting   24. Cell-penetrating peptides as carriers for transepithelial drug delivery Danai Anastasia Panou, Ragna Guldsmed Diedrichsen, Mie Kristensen, and Hanne Mørck Nielsen   25. New tools for streamlined in vivo homing peptide identification Kristina Põšnograjeva, Karlis Pleiko, Maarja Haugas, and Tambet Teesalu         26. Tumor targeting as new therapeutic approach Eric Savier, Pierre Tuffery, Heriberto Bruzzoni-Giovannelli and Angelita Rebollo   27. Mitochondrial Targeting Probes, drug conjugates and gene therapeutics Carmine Pasquale Cerrato, Tove Kivijärvi, and Ülo Langel   28. Novel brain penetrating single chain antibodies directed against 3RTau for the Treatment of Alzheimer’s Disease and related Dementias Brian Spencer, Robert A. Rissman, Cassia Overk, and Eliezer Masliah   29. Strategies for peptide-mediated cargo delivery to human smooth muscle cells Leo Gurney, Steven C. Robson, Michèle Sweeney, Arwyn T. Jones, and Michael J. Taggart   Part IV: Oligonucleotide delivery   30. Tips and Tools to Understand Direct Membrane Translocation of siRNA-Loaded WRAP-Based Nanoparticles. Sébastien Deshayes, Karidia Konate, Eric Vivès, and Prisca Boisguérin   31. Evaluating efficacy of peptide-delivered oligonucleotides using the severe Taiwanese SMA mouse model Larissa Goli, Jessica Stoodley, Suzan Hammond, and Richard Raz   32. Development of CPP-based methods for delivery of miRNAs into the skin and airways - lessons from cell culture and mouse models Anet Laanesoo, Kapilraj Periyasamy, Margus Pooga, and Ana Rebane   33. Endpoint and kinetic approaches for assessing transfection efficacy in mammalian cell culture  Ly Porosk, Jekaterina Nebogatova, Ilja Gaidutšik, and Ülo Langel   34. Tissue analysis of lung-targeted delivery of siRNA and plasmid DNA Kaido Kurrikoff, Birgit Vunk, and Ülo Langel   35. Improvement of transfection with PepFects by organic and inorganic components. Moataz Dowaidar, Hani Nasser Abdelhamid, and Ûlo Langel   36. DNA Transfer into Animal Cells Using Stearylated CPP Based Transfection Reagent Kristi Kasemaa, Kaia Talts, Evelin Täht, Urve Toots, and Mart Ustav   37. Use of PepFect14 and siRNA for targeted gene silencing in human embryonic stem cells Egle-Helene Ervin, Margus Pooga, and Toivo Maimets   38. CRISPR/Cas9 plasmid delivery through the CPP: PepFect14 Luca Falato, Birgit Vunk, and Ülo Langel   39. Applications of CPPs in genome editing of plants Atta Soliman, John Laurie, Andriy Bilichak, and Alicja Ziemienowicz   40. Live Cell Genomics: Cell-specific transcriptome capture and exon-specific RNA binding protein isolation in live tissues and cells Jaehee Lee and James Eberwine

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