Overview

This detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration. The book focuses on the cell biology of cell migration, developmental model systems to assess cell migration during morphogenesis, cell migration in cancers and the tumor micro-environment, as well as blood vessel formation and interactions. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Cell Migration in Three Dimensions provides a solid foundation for scientists of different disciplines to investigate cell migration in biological processes.  Chapters 7, 12, 16, 17, 19, 22, and 24 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Full Product Details

Author:   Coert Margadant
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2608
Weight:   1.107kg
ISBN:  

9781071628867

ISBN 10:   1071628860
Pages:   472
Publication Date:   19 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Cell Migration in Three Dimensions             Coert Margadant   Part I: The Cell Biology of Cell Migration   2. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis             Clotilde Huet-Calderwood, Felix Rivera-Molina, Derek Toomre, and David A. Calderwood   3. Probing the ER-Focal Adhesion Link during Cell Migration             Noemi Antonella Guadagno and Cinzia Progida   4. Mapping the Localization of Proteins within Filopodia Using FiloMap             Guillaume Jacquemet   5. Extended Methods for 2D Confinement             Juan Manuel García-Arcos, Kevin Gateau, Larisa Venkova, and Matthieu Piel   6. Visualization of Exosome Release and Uptake during Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63             Bong Hwan Sung and Alissa M. Weaver   7. Approaches to Determine Nuclear Shape in Cells during Migration through Collagen Matrices             Martin Svoren, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, and Katarina Wolf   Part II: Developmental Model Systems to Assess Cell Migration during Morphogenesis   8. Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila             Maik C. Bischoff and Sven Bogdan   9. Whole Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells             Antonius L. van Boxtel   10. Live Imaging Analysis of Epithelial Zippering during Mouse Neural Tube Closure             Matteo A. Molè, Gabriel L. Galea, and Andrew J. Copp   11. Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila             Sofia J. Araújo and Marta Llimargas   12. A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland             Edouard Hannezo and Colinda L.G.J. Scheele   13. Analysis of Integrin-Dependent Melanoblast Migration during Development             Amanda Haage and Guy Tanentzapf   Part III: Cell Migration in Cancers and the Tumor Microenvironment   14. Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices             David Remy, Anne-Sophie Macé, Philippe Chavrier, and Pedro Monteiro   15. Analysis of Energy-Driven Leader-Follower Hierarchy during Collective Cancer Cell Invasion             Jian Zhang and Cynthia A. Reinhart-King   16. Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids             Konstadinos Moissoglu, Stephen J. Lockett, and Stavroula Mili   17. Multimodal Techniques to Study Tumor Growth, Basement Membrane Breaching, and Invasion in 3D Matrices             Daan Smits and Antoine A. Khalil   18. Analysis of Collective Migration Patterns within Tumors             Ralitza Staneva and Andrew G. Clark   19. An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-Evaluation             Ann L.B. Seynhaeve and Timo L.M. ten Hagen   Part IV: Blood Vessel Formation and Interactions   20. Angiogenesis Invasion Assay to Study Endothelial Cell Invasion and Sprouting Behavior             Yuechao Dong, Florian Alonso, Tiya Jahjah, Isabelle Fremaux, and Elisabeth Génot   21. Live-Cell Labeling and Artificial Intelligence Approaches for High-Resolution XYZT Imaging of Cytoskeletal Dynamics during Collective Cell Migration             Maxime Cammeraat, Marko Popovic, Wendy Stam, and Coert Margadant   22. Analysis of mRNA Subcellular Distribution in Collective Cell Migration             Joshua J. Bradbury, Holly E. Lovegrove, Marta Giralt-Pujol, and Shane P. Herbert   23. A Bio-Engineered Model for Studying Vascular-Pericyte Interactions of the Placenta             Marta Cherubini and Kristina Haase   24. Analysis of Vascular Morphogenesis in Zebrafish             Marvin Hubert and Wiebke Herzog   25. Analysis of Monocyte Recruitment during Inflammation by Intravital Imaging             Jose M. Gonzalez-Granado, Alberto Del Monte, Laura Piqueras, Vicente Andres, and Cristina Rius

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