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Author:   Anthony W. Ryan
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2015
Volume:   1326
Dimensions:   Width: 17.80cm , Height: 2.00cm , Length: 25.40cm
Weight:   6.706kg
ISBN:  

9781493928385

ISBN 10:   1493928384
Pages:   235
Publication Date:   24 October 2015
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
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Table of Contents

Celiac Disease: Background and Historical Context.- Celiac Disease: Diagnosis.- Generating Transgenic Mouse Models for Studying Celiac Disease.- Study Designs for Exploring the Non-HLA Genetics in Celiac Disease.- 24 Hour Ex Vivo Culture of Coeliac Duodenal Biopsies.- Isolation and Cloning of Gluten-Specific T Cells in Celiac Disease.- Flow Cytometric Analysis of Human Small Intestinal Lymphoid Cells.- Adaptation of a Cell-Based High Content Screening System for the In-depth Analysis of Celiac Biopsy Tissue.- HLA Genotyping: Methods for the Identification of the HLA-DQ2, -DQ8 Heterodimers Implicated in Celiac Disease (CD) Susceptibility.- Detecting Allelic Expression Imbalance at Candidate Genes Using 5' Nuclease Genotyping Technology.- Gene Expression Profiling of Coeliac Biopsies and Peripheral Blood Monocytes Using Taqman Assays.- Cloning Gene Variants and Reporter Assays.- Epigenetic Methodologies for the Study of Celiac Disease.- Candidate Gene Knockdown in Celiac Disease.- Perl One-Liners: Bridging the Gap Between Large Data-Sets and Analysis Tools.-  Bioinformatic Analysis of Antigenic Proteins.- Quality Control Procedures for Genome Wide Association Studies using the Immunochip Platform.- Quality Control and Analysis of NGS RNA Sequencing Data.

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Author Information

Anthony W. Ryan PhD Room 1.17, Institute of Molecular Medicine Trinity Centre for Health Sciences Trinity College Dublin St James's Hospital, Dublin 8, Ireland. tel +353 1 896 3273 (lab 896 3292) fax +353 1 454 2043 e-mail aryan12@tcd.ie

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