Overview

This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Cartilage Tissue Engineering serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair.

Full Product Details

Author:   Martin J. Stoddart ,  Elena Della Bella ,  Angela R. Armiento
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2598
Weight:   0.969kg
ISBN:  

9781071628386

ISBN 10:   1071628380
Pages:   393
Publication Date:   11 November 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Cartilage Tissue Engineering: An Introduction             Martin J. Stoddart, Elena Della Bella, and Angela R. Armiento   2. Chondrocyte Isolation and Expansion             Paul Harrison, Timothy Hopkins, Charlotte Hulme, Helen McCarthy, and Karina Wright   3. Isolation of Chondrons from Hyaline Cartilage             Jasmijn V. Korpershoek, Margot Rikkers, and Lucienne A. Vonk   4. Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation             Ilyas M. Khan, Joshua McKenna, and Yadan Zhang   5. Physioxic Culture of Chondrogenic Cells             Girish Pattappa, Brandon D. Markway, Denitsa Docheva, and Brian Johnstone   6. Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells             Angela R. Armiento, Yann D. Ladner, Elena Della Bella, and Martin J. Stoddart   7. Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation             Justyna M. Buchert, Benedict Lotz, Solvig Diederichs, and Wiltrud Richter   8. Chondrogenic Differentiation of Human Induced Pluripotent Stem Cells             Amanda R. Dicks, Nancy Steward, Farshid Guilak, and Chia-Lung Wu   9. The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulphated Glycosaminoglycans             Yann D. Ladner, Mauro Alini, and Angela R. Armiento   10. Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy             Alexander Otahal, Andrea De Luna, Ali Mobasheri, and Stefan Nehrer   11. Metabolomic Profiling to Understand Chondrocyte Metabolism             Priyanka P. Brahmachary, Hope D. Welhaven, and Ronald K. June   12. Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes             Huan Meng, Clare L. Thompson, Clarissa Coveney, Angus K. Wann, and Martin M. Knight   13. Co-Culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads             Teresa Z. Brose, Yann D. Ladner, E. Johanna Kubosch, Martin J. Stoddart, and Angela R. Armiento   14. Isolation of Murine Articular Chondrocytes for Single Cell RNA or Bulk RNA Sequencing Analysis             Jillian L. McCool, Nicholas R. Hum, Aimy Sebastian, and Gabriela G. Loots   15. Modulation of MicroRNA Expression during In Vitro Chondrogenesis             Austin Bell-Hensley, Hongjun Zheng, and Audrey McAlinden   16. Purification and Isolation of Proteins from Hyaline Cartilage             Makenna J. Hardy, Xinzhu Pu, and Julia Thom Oxford   17. Histological Preparation and Evaluation of Cartilage Specimens             Salim E. Darwiche, Milena Tegelkamp, Katja Nuss, and Brigitte von Rechenberg   18. Articular Cartilage Imaging in the Context of the Superficial Chondrocyte Spatial Organization (SCSO) as a Surrogate Marker for Functional Pathology             Bodo Kurz, Thomas Lange, Marita Voelker, and Bernd Rolauffs   19. Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization             Seyed Ali Elahi, Rocío Castro-Viñuelas, Anke Govaerts, Rik Lories, Nele Famaey, and Ilse Jonkers   20. Viral Gene Delivery in Chondrocytes             Christopher V. Nagelli, Christopher H. Evans, and Rodolfo E. De La Vega   21. Decellularization of Articular Cartilage: A Hydrochloric Acid-Based Strategy             Cornelia Schneider and Sylvia Nürnberger   22. Self-Assembly Culture Model for Engineering Musculoskeletal Tissues             Nicholas N. DePhillipo, Jerahme Martinez, and George R. Dodge   23. Osteochondral Explants Isolation and Culture under a Compression and Shear Bioreactor             M. Letizia Vainieri and Sibylle Grad   24. A Mouse Model of Acute Cartilage Injury and Repair             Anne-Sophie Thorup, Francesco Dell'Accio, and Suzanne E. Eldridge   25. Small-Animal Compression Models of Osteoarthritis             Blaine A. Christiansen, Deva D. Chan, Marjolein C.H. van der Meulen, and Tristan Maerz   26. Modelling and Assessing Osteoarthritis in Mice by Destabilization of the Medial Meniscus (DMM)             Jadwiga Miotla-Zarebska, Ida Parisi, Vicky Batchelor, Tonia L. Vincent, and Paul K. Potter   27. Collagenase-Induced Osteoarthritis in Mice             Peter M. van der Kraan   28. Platelet-Rich Plasma for Intra-Articular Injections: Preclinical and Clinical Evidence             Angelo Boffa and Giuseppe Filardo

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