Overview

An indispensable collection of updated classical and emerging techniques that promise to add critical knowledge to our understanding of cartilage metabolism in health and disease. Volume 1: Cellular and Molecular Tools describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for studies - at the tissue level - of turnover, structure, and functioning of normal and diseased cartilage by invasive and noninvasive means. Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand - from the cellular to the animal level - the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.

Full Product Details

Author:   Massimo Sabatini ,  Philippe Pastoureau ,  FrTdTric De Ceuninck
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2004 ed.
Volume:   100
Dimensions:   Width: 15.50cm , Height: 2.20cm , Length: 23.50cm
Weight:   0.798kg
ISBN:  

9781588292476

ISBN 10:   1588292479
Pages:   358
Publication Date:   28 July 2004
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Culture and Phenotyping of Chondrocytes in Primary Culture.- Culture of Chondrocytes in Alginate Beads.- Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines.- Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function.- Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype.- Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR.- Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies.- RNA Extraction From Cartilage.- Gene Expression Analysis in Cartilage by In Situ Hybridization.- Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis.- High-Efficiency Nonviral Transfection of Primary Chondrocytes.- In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors.- Changes of Chondrocyte Metabolism In Vitro.- Analysis of Chondrocyte Functional Markers and Pericellular Matrix Components by Flow Cytometry.- A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes.- Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants.- Production of Antibodies Against Degradative Neoepitopes in Aggrecan.- Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures.- Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes.- Expression, Activity, and Regulation of MAP Kinases in Cultured Chondrocytes.- Mechanical Loading of Chondrocytes Embedded in 3D Constructs.- In Vitro Physical Stimulation of Tissue-Engineered and Native Cartilage.

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