Overview

The advances in molecular biology and genetics coupled with the progress in instrumentation and surgical techniques have produced a voluminous and often bewildering quantity of data. The primary objective of this second edition is to critically interpret the literature and to provide a framework for the enormous amount of information in this burgeoning field. As in the first edition, the monograph serves as a practical guide for the investigator interested in the functional methods used to characterize the murine cardiovascular phenotype. The monograph is organized into three parts. The first deals with principles of transgenesis and homologous recombination. The second part, which again is the largest, discusses the various techniques used to assess the cardiovascular mechanical, metabolic, and electrophysiologic phenotype. This section is organized in a hierarchical manner - i.e. from isolated myocyte to isolated heart to the intact, anesthetized and conscious mouse. The third part examines techniques used to evaluate murine smooth muscle function, genetic mouse models of hypertrophy and heart failure, and the methods to assess the cardiovascular phenotype in the developing mouse embryo. In addition, newer methods that ""push the envelope"", such as magnetic resonance imaging (MRI), positron emission tomography (PET), ultrasound biomicroscopy (UBM), and computed tomography (microCT) are discussed. Expanded and updated, each chapter is richly enhanced with original tables and figures, and in many cases, extensively rewritten when compared with the first edition. An essential and enduring goal of this second edition is to continue to facilitate interactions between the basic science disciplines and help bridge the gap between molecular biology and physiology.

Full Product Details

Author:   Brian D. Hoit ,  Richard A. Walsh
Publisher:   Springer
Imprint:   Springer
Edition:   2nd ed. 2002
Volume:   238
Dimensions:   Width: 15.50cm , Height: 2.30cm , Length: 23.50cm
Weight:   1.690kg
ISBN:  

9780792375364

ISBN 10:   079237536
Pages:   403
Publication Date:   31 October 2001
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

One.- 1. The Hierarchical Approach to Analysis of Cardiovascular Function in Mice: An Overview.- 2. Genetic Alterations and Modeling of Cardiovascular Physiology.- 3. Conditional Transgenesis.- 4. Array Transcription Profiling: Molecular Phenotyping Of Rodent Cardiovascular Models.- 5. Murine Cardiovascular Development.- Two.- 6. Isolated Myocyte Mechanics and Calcium Transients.- 7. Single Cell Patch-Clamp Analysis of Mouse Cardiac Myocytes.- 8. The Isolated Isovolumic (Langendorff) Heart Preparation.- 9. The Isolated Work-Performing and Ejecting Mouse Heart Preparation, Comparison and Quantification of Cardiac Performance in Transgenic and Wild-type Mice.- 10. 31P NMR Spectroscopy of the Mouse Heart.- 11. Electrophysiological Characterization of the Mouse Heart Using Voltage-Sensitive Dyes.- 12. Echocardiographic Assessment of the Mouse Heart and Aorta.- 13. In Vivo Invasive Hemodynamic Studies in Mice.- 14. Pressure-Volume Relations.- 15. Assessment of Left Ventricular Diastolic Function in Mice.- 16. In Vivo Electrophysiologic Studies in Mice.- 17. Cardiovascular Physiology in Mice: Conscious Function Measurements and Effects of Anesthesia.- 18. Exercise and Oxygen Consumption in the Mouse.- 19. Clinical Laboratory Analysis of the Genetically Manipulated Mouse.- Three.- 20. Smooth Muscle Studies Using Gene-Altered Mouse Models: A Users’ Guide.- 21. Models of Cardiac Disease in the Mouse, Cardiac Hypertrophy and Heart Failure.- 22. Analysis of Murine Embryonic Cardiovascular Phenotype.- 23. Newer In Vivo Imaging Modalities.

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