Overview

This second edition brings together a variety of method used in the field of cardiovascular development research. Chapters guide readers through protocols on lymphatic development, lymphatic staining techniques, lymphatic endothelial, tissue clearing techniques, and muscle cell isolation and culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls Authoritative and practical, Cardiovascular Development: Methods and Protocols, Second Edition seeks to aid scientists in understanding new state-of-the-art techniques in the field of cardiovascular development research.

Full Product Details

Author:   Xu Peng ,  Warren E. Zimmer
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2319
Weight:   0.339kg
ISBN:  

9781071614822

ISBN 10:   1071614827
Pages:   162
Publication Date:   31 July 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

  1. Tissue clearing and 3-D visualization of Vasculature with the PEGASOS Method Dian Jing, Yi Men, and Hu Zhao   2. Preparation and Identification of Cardiac Myofibrils from Whole Heart Sample Heidi Creed and Carl W. Tong   3. RNA-Sequencing and Transcriptome Analysis after Fluid Shear Stress Stimulation in Lymphatic Endothelial Cells Hongjiang Si and Xu Peng   4. Preparation of Neonatal Rat Papillary Muscles for Contractile Studies Steven Jokerst, Damir Nizmutdinov, Charley Edgar, April M. Kaspick, Carl W. Tong, and David E. Dostal   5. In Vitro Study of permeability in Lymphatic Endothelial Cells Responding to Histamine Hongjiang Si   6. Optimizing the Differentiation of Cardiomyocytes from Human Induced Pluripotent Derived-Stem Cells Melanie Gartz and Jennifer L. Strande     7. Analysis of Lymphatic Vessel Formation by Whole-mount Immunofluorescence Staining Jian Wang, Yuwei Dong, Mariappan Muthuchamy, David C. Zawieja, and Xu Peng   8. Isolation and Culture of Mouse Lymphatic Endothelial Cells from Lung Tissue Philip E. Lapinski and Philip D. King   9. Laser-induced Choroidal Neovascularization in Rats       Min Zhao, Wankun Xie, Travis W. Hein, Lih Kuo, and Robert H. Rosa, Jr.   10. Visualizing Three-Dimensional Vascular Networks in the Mouse Embryonic Hindbrain Chenshen Huang, Liangjing Wu, Jian Wang, Binu Tharakan, and Xu Peng   11. Visualizing Blood Vessel Development in Cultured Mouse Embryos Using Lightsheet Microscopy Samantha J. Fredrickson, Tanner G. Hoog, and Ryan S. Udan   12. Laser Capture Microdissection of Vascular Endothelial Cells from Frozen Heart Tissues Tianhao Zhou and Jian Wang   13. Visualization of Fundus Blood Vessels Wankun Xie, Min Zhao, Travis W. Hein, Lih Kuo, and Robert H. Rosa, Jr.   14. Transient Transgenics: An Efficient Method to Identify Gene Regulatory Elements Warren E. Zimmer and Robert J. Schwartz   15. Isolation of Lymphatic Muscle Cells (LMCs) from Rat Mesentery Xueyang Zhang, Sanjukta Chakraborty, Mariappan Muthuchamy, and David C. Zawieja   16. Isolation of Adult Mouse Cardiomyocytes using Langendorff Perfusion Apparatus Yang Liu, David E. Dostal, and Carl W. Tong   17. Visualization of Angiogenesis in Mouse Embryonic Dorsal Skin by Whole-Mount Fluorescent Staining Jian Wang, Yuwei Dong, Yu Xi, and Xu Peng

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