Overview

This detailed volume presents an updated collection of state-of-the-art protocols in cardiac tissue engineering. These protocols demonstrate advancements in cell sourcing, assembly, and use of engineered cardiac tissues, imaging and diagnostics, as well as therapeutic applications. New animal models, biomaterials, and quantitative analyses are described for broad adoption. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Cardiac Tissue Engineering: Methods and Protocols, Second Edition serves as an ideal resource for inspiring the advancement of cardiotoxicity assessment, drug discovery, and heart repair and regeneration in order to accelerate heart health improvement around the globe.

Full Product Details

Author:   Kareen L.K. Coulombe ,  Lauren D. Black III
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2485
Weight:   0.823kg
ISBN:  

9781071622605

ISBN 10:   1071622609
Pages:   314
Publication Date:   28 May 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. CRISPR Library Screening in Cultured Cardiomyocytes             Sophia DeLuca and Nenad Bursac   2. Protein and mRNA Quantification in Small Samples of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well Microplates             Weizhen Li, Julie L. Han, and Emilia Entcheva   3. Self-Assembled Heterotypic Cardiac Spheroids from Human Pluripotent Stem Cells             Oriane B. Matthys and Todd C. McDevitt   4. Acellular Myocardial Scaffolds and Slices Fabrication, and Method for Applying Mechanical and Electrical Simulation to Tissue Construct             Bo Wang, Mickey Shah, Lakiesha N. Williams, Amy L. de Jongh Curry, Yi Hong, Ge Zhang, and Jun Liao   5. FRESH 3D Bioprinting a Ventricle-Like Cardiac Construct Using Stem Cell-Derived Cardiomyocytes             Brian D. Coffin, Andrew R. Hudson, Andrew Lee, and Adam W. Feinberg   6. Engineered Heart Tissues for Contractile, Structural, and Transcriptional Assessment of Human Pluripotent Stem Cell-Derived Cardiomyocytes in a Three-Dimensional, Auxotonic Environment             Samantha Bremner, Alex J. Goldstein, Ty Higashi, and Nathan J. Sniadecki   7. High-Throughput Analysis of Drug Safety Responses in Induced Pluripotent Stem Cell-Derived Cardiomyocytes Using Multielectrode Array             Nadjet Belbachir, Nathan Cunningham, and Joseph C. Wu   8. iPSC-Derived Micro-Heart Muscle for Medium Throughput Pharmacology and Pharmacogenomic Studies Daniel W. Simmons and Nathaniel Huebsch   9. Quantifying Propagation Velocity from Engineered Cardiac Tissues with High-Speed Fluorescence Microscopy and Automated Analysis Software             Andrew P. Petersen and Megan L. McCain   10. Arrhythmia Assessment in Heterotypic Human Cardiac Myocyte–Fibroblast Microtissues             Celinda M. Kofron, Bum-Rak Choi, and Kareen L.K. Coulombe   11. Human Engineered Atrial Tissue for Studying Atrial Fibrillation Julia Krause, Marta Lemme, Ingra Mannhardt, Alexandra Eder, Bärbel Ulmer, Thomas Eschenhagen, and Justus Stenzig   12. Design and Fabrication of Biological Wires for Cardiac Fibrosis Disease Modeling             Erika Yan Wang, Jacob Smith, and Milica Radisic   13. Methods for Transepicardial Cell Transplantation in a Swine Myocardial Infarction Model             Fanny Wulkan, Rocco Romagnuolo, Beiping Qiang, and Michael A. Laflamme   14. Defined Engineered Human Myocardium for Disease Modeling, Drug Screening, and Heart Repair             Malte Tiburcy, Tim Meyer, Pierre-Luc Satin, and Wolfram-Hubertus Zimmermann   15. Tubular Cardiac Tissue Bioengineered from Multi-Layered Cell Sheets for Use in the Treatment of Heart Failure             Hidekazu Sekine and Teruo Okano   16. Quantifying Cardiomyocyte Proliferation and Nucleation to Assess Mammalian Cardiac Regeneration             Emma B. Brandt and Ahmed I. Mahmoud   17. Injectable ECM Scaffolds for Cardiac Repair             Jervaughn D. Hunter, Todd D. Johnson, Rebecca L. Braden, and Karen L. Christman   18. Encapsulation of Pediatric Cardiac-Derived C-Kit+ cells in Cardiac Extracellular Matrix Hydrogel for Echocardiography-Directed Intramyocardial Injection in Rodents             Preety Shakya, Milton E. Brown, and Michael E. Davis   19. Characterization of the Monocyte Response to Biomaterial Therapy for Cardiac Repair             Sarah McLaughlin, David Smyth, Emilio I. Alarcon, and Erik J. Suuronen   20. Right Ventricular Outflow Tract Surgical Resection in Young, Large Animal Model for the Study of Alternative Cardiovascular Patches             Elizabeth C. Porter, Whitney L. Stoppel, Raymond K. Kudej, and Lauren D. Black III

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