Overview

A compilation of modern hands-on methods using microbes and enzymes to produce and modify carbohydrates of potential and actual commercial value. These powerful methods enable both the expert and the beginner to generate polysaccharides, oligosaccharides, and carbohydrate-based surfactants by fermentation using enzymes. Additional techniques make it possible to produce derivatives of sugars, other oligosaccharides, and sugar derivatives using enzyme technology. Carbohydrate Biotechnology Protocols offers synthetic chemists, biochemists, fermentation technologists, and applied enzymologists cutting-edge techniques - many of them hitherto unavailable - that are cleaner and often less costly than available chemical alternatives.

Full Product Details

Author:   Christopher Bucke
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1999 ed.
Volume:   10
Dimensions:   Width: 15.50cm , Height: 2.30cm , Length: 23.50cm
Weight:   1.520kg
ISBN:  

9780896035638

ISBN 10:   0896035638
Pages:   337
Publication Date:   23 July 1999
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

to Carbohydrate Biotechnology.- Production and Isolation of Xanthan Gum.- Alginate from Azotobacter vinelandii.- Production of Schizophyllan.- Enzymatic Synthesis of Cellulose.- Modification of Alginate Using Mannuronan C-5-Epimerases.- Viscosity Control of Guar Polysaccharide Solutions by Treatment with Galactose Oxidase and Catalase Enzymes.- The Production of Cyclodextrins Using CGTase from Bacillus macerans.- Production of Microbial Glycolipids.- Partial Enzymatic Hydrolysis of Starch to Maltodextrins on the Laboratory Scale.- The Production of ?(1?2)-Terminated Glucooligosaccharides.- Enzymatic Production of Fructooligosaccharides from Sucrose.- Enzymatic Production of Inulooligosaccharides from Inulin.- One-Pot Enzymatic Synthesis of Sialyl T-Epitope.- Hydrolysis of Hemicelluloses Using Combinations of Xylanases and Feruloyl Esterases.- Enzymatic Depolymerization of Chitins and Chitosans.- Synthesis of Homo- and Hetero-Oligosaccharides from Underivatized Sugars Using Glycosidases.- Use of Fluorophore-Assisted Carbohydrate Electrophoresis (FACE®) in the Elucidation of N-Linked Oligosaccharide Structures.- Application of Sucrose Synthase in the Synthesis of Nucleotide Sugars and Saccharides.- Production of Isomaltulose Using Immobilized Bacterial Cells.- The Production of Mannitol by Fermentation.- The Production of 3-Keto-Derivatives of Disaccharides.- Enzymatic Synthesis of ?-Butylglucoside in a Biphasic Butanol-Water System Using the ?-Transglucosidase from Aspergillus niger.- Enzymatic Glycosylation of Aglycones of Pharmacological Significance.- Enzymatic Synthesis of Glycosides in Aqueous-Organic Two-Phase Systems and Supersaturated Substrate Solutions.- Use of ?-Glucosidase in the Development of Flavor in Wines and Fruit Juices.

Reviews

...The recommendable book is very useful for students to start experiment work and for scientists and practitioners in doing experimental tasks. - Nahrung the individual chapters are well written by subject matter authorities, and provide useful insights to the original research literature. The bench manual format of the book perhaps makes it most useful to active researchers interested in the specific protocols selected for this volume, although a wealth of interesting information is found among the chapter introductions and notes. - ASM News Each chapter of the volume is armed with necessary literature references, as basic sources of knowledge. The contributions throughout the book are easily readable and understandable, this is why I recommend the book not only to experts in synthetic chemistry, biochemistry, fermentation biotechnology, and applied enzymology, but also to beginners and students who are new to the field of carbohydrate biotechnology. - Models of Chemistry The protocols are clearly arranged and begin with a short theoretical and commercial background of the method, the list of reagents and equipment and continue with a comprehensive step-by-step description and a set of notes. Such procedure saves time of the user, indicating quickly which problems are likely to arise. The recommendable book is very useful for students to start experimental work and for scientists and practitioners in doing experimental tasks. - Nahrung a valuable resource to more-experienced practitioners who require alternative methodologies. this volume will prove a useful resource for researchers of graduate level and above, with an interest in investigating carbohydrate biochemistry as applied to the fields of biotechnology and pharmacology. - Tibtech

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