Overview
Capillary Electrophoresis (CE) provides researchers with the ability to separate and analyze very small amounts of DNA. CE offers important advantages over other available methods because of the speed and reproducibility of analysis. further, the high sensitivity of detection and the ability to increase sample throughput with parallel analysis has led to the creation of a full range of techniques for the analysis of DNA molecules, from modified DNA-adducts and single strand oligonucleotides through to PCR-amplified DNA fragments and whole chromosomes. Capillary Electrophoresis of Nucleic Acids provides these analytical protocols, which can be used for detection and analysis of mutations and modifications to precise DNA loci through to entire genomes of organisms. Volume II of this two-volume set broadly addresses techniques for high-throughput analysis of DNA fragments of less than 1 kbm employing SNP detection, mutation detection, DNA sequencing methods, and DNA ligand interactions. It presents practical considerations necessary for very rapid and accurate separations of linear DNAs by CE using short capillaries. Also covered are the various modes of genotyping by CE with an emphasis on the use of multiplex separations followed by techniques for the quantitative estimation of gene suppression using CE.
Full Product Details
Author: Keith R. Mitchelson ,
Jing Cheng
Publisher: Humana Press Inc.
Imprint: Humana Press Inc.
Edition: 2001 ed.
Volume: 163
Dimensions:
Width: 15.20cm
, Height: 2.40cm
, Length: 22.90cm
Weight: 0.851kg
ISBN: 9780896037656
ISBN 10: 0896037657
Pages: 408
Publication Date: 19 January 2001
Audience:
College/higher education
,
Professional and scholarly
,
Undergraduate
,
Professional & Vocational
Format: Hardback
Publisher's Status: Active
Availability: Out of stock
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.
Reviews
These volumes comprehensively and accurately present CE technology and techniques with an emphasis on providing an understanding of the theory and instrumentation (Vol. 1), as well as detailed examples of successful analytical protocols (both volumes)...Chapters on the use of microchip and mass spectrometric detection are also included...Although numerous experimental protocols can be found in Vol. 1, it is clear that the intent is to communicate the underlying theory and capability of CE for separations. Vol. 1 should be of interest to the novice as well as the expert. In Vol. 2, each of the 31 chapters present specific examples of the use of CE. ...Nonetheless, these two volumes of the Methods in Molecular Biology series offer a representative and needed overview of the current theory and practice of CE of nucleic acids. These texts should prove very useful to both active investigators in this area and those seeking to learn more about the use and and capability of CE. - Clinical Chemistry
These volumes comprehensively and accurately present CE technology and techniques with an emphasis on providing an understanding of the theory and instrumentation (Vol. 1), as well as detailed examples of successful analytical protocols (both volumes). Chapters on the use of microchip and mass spectrometric detection are also included. Although numerous experimental protocols can be found in Vol. 1, it is clear that the intent is to communicate the underlying theory and capability of CE for separations. Vol. 1 should be of interest to the novice as well as the expert. In Vol. 2, each of the 31 chapters present specific examples of the use of CE. Nonetheless, these two volumes of the Methods in Molecular Biology series offer a representative and needed overview of the current theory and practice of CE of nucleic acids. These texts should prove very useful to both active investigators in this area and those seeking to learn more about the use and and capability of CE. - Clinical Chemistry
