Overview

A collection of cutting-edge techniques for using capillary electrophoresis (CE) to analyze complex carbohydrates. These readily reproducible protocols provide methods for sample preparation, analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and olgosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, notes, comments, and tips on procedures.

Full Product Details

Author:   Pierre Thibault ,  Susumu Honda
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2003
Volume:   213
Dimensions:   Width: 15.20cm , Height: 1.80cm , Length: 22.90cm
Weight:   0.510kg
ISBN:  

9781617372063

ISBN 10:   1617372064
Pages:   318
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Structural and Functional Diversity of Glycoconjugates.- Sample Preparation Methods.- Chemical and Enzymatic Release of Glycans from Glycoproteins.- Derivatization of Carbohydrates.- Preparation of Oligosaccharides from Sulfated Glycosaminoglycans Using Bacterial Enzymes.- Analysis of Mono- and Oligosaccharides.- Monosaccharide Analysis by Capillary Electrophoresis.- Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers.- Characterization of Oligosaccharides from Starch, Dextran, Cellulose, and Glycoproteins by Capillary Electrophoresis.- Affinity Capillary Electrophoresis of Oligosaccharides.- Characterization of Glycosaminoglycans by Capillary Electrophoresis.- Analysis of Glycoconjugates and the Component Monosaccharides.- Analysis of 2-Aminoacridone-Derivatized Complex Oligosaccharides Using Micellar Electrokinetic Capillary Chromatography and Laser-Induced Fluorescence Detection.- Separation of Protein Glycoforms by Capillary Electrophoresis.- Separation of Glycoproteins by Capillary Isoelectric Focusing.- Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry.- Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry.- Specific Applications.- Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity.- Determination of Association Constant of Carbohydrate-Protein Interaction.

Reviews

...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches. -Pharmaceutical Research ...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field. -Biomedical Chromatography ...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems. - Chromatographia ...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis. - Biochemistry

"""...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches."" -Pharmaceutical Research ""...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field."" -Biomedical Chromatography ""...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems."" - Chromatographia ""...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis."" - Biochemistry"

...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches. -Pharmaceutical Research ...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field. -Biomedical Chromatography ...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems. - Chromatographia ...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis. - Biochemistry

...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches. -Pharmaceutical Research ...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field. -Biomedical Chromatography ...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems. - Chromatographia ...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis. - Biochemistry

...the book will be very valuable for researchers carrying out carbohydrate research, or planning to do so, and contemplating the best analytical approaches. -Pharmaceutical Research ...this book provides a great deal of useful practical detail for CE analysis of carbohydrates for the experienced as well as the newcomer to the field. -Biomedical Chromatography ...the book is a nice compilation of protocols for the analysis of carbohydrates by CE...it does contain all the essential procedures and methods needed to solve many problems. - Chromatographia ...this book comprehensively summarizes knowledge on carbohydrate analysis by means of capillary electrophoresis. - Biochemistry

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