Overview

This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.

Full Product Details

Author:   Christian Neusüß ,  Kevin Jooß
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2531
Weight:   0.714kg
ISBN:  

9781071624920

ISBN 10:   107162492
Pages:   260
Publication Date:   09 August 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Evaluation of prototype CE-MS interfaces Sabrina Ferré, Julien Boccard, Serge Rudaz, and Víctor González-Ruiz   2. Capillary Electrophoresis-Mass Spectrometry (CE-MS) by Sheath-Flow Nanospray interface and its use in biopharmaceutical applications Mei Han, Richard Smith, and Dan A. Rock   3. Protocol for etching bare-fused silica capillaries for sheathless capillary electrophoresis-mass spectrometry coupling Clarisse Gosset-Erard, Jérémie Giorgetti, Michael Biacchi, Frédéric Aubriet, Emmanuelle Leize-Wagner, Patrick Chaimbault, and Yannis-Nicolas François   4. Microfabricated Liquid Junction Capillary Electrophoresis-Mass Spectrometry Interface Jana Krenkova and Frantisek Foret   5. Successive Multiple Ionic-Polymer Layer Coatings for intact protein analysis by Capillary Zone Electrophoresis - Mass Spectrometry – Application to Hemoglobin Analysis Liesa Salzer, Alexander Stolz, Laura Dhellemmes, Alisa Höchsmann, Laurent Leclercq, Hervé Cottet, and Christian Neusüß   6. On-line Immobilized Enzyme Microreactor Capillary Zone Electrophoresis-Mass Spectrometry for Peptide Mapping Roger Pero-Gascon, Laura Pont, Estela Giménez, Victoria Sanz-Nebot and Fernando Benavente 7. Bottom up analysis of proteins by peptide mass fingerprinting with tCITP-CZE-ESI-TOF MS after tryptic digest Marius Segl and Hanno Stutz   8. Top-down proteomics by capillary zone electrophoresis-tandem mass spectrometry for large-scale characterization of proteoforms in complex samples Elijah N. McCool, Rachele A. Lubeckyj, Daoyang Chen, and  Liangliang Sun   9. Assessment of macro- and microheterogeneity of monoclonal antibodies using Capillary Zone Electrophoresis hyphenated with Mass Spectrometry Christoph Gstöttner, Rob Haselberg, Manfred Wuhrer, Govert W. Somsen, Elena Domínguez-Vega   10. High Sensitivity Glycoproteomic Analysis of Biological Samples by CZE-ESI-MS Wei Wang and Guinevere S.M. Lageveen-Kammeijer   11. Capillary Zone Electrophoresis-Electrospray Ionization Tandem Mass Spectrometry for Total Analysis of Chondroitin/Dermatan Sulfate Oligosaccharides Alina D. Zamfir   12. In-Source Fragmentation for Identification of Compounds by CE-ESI-TOF in Human Plasma. L-Proline as case study Maricruz Mamani-Huanca, Ana Gradillas, Ángeles López-Gonzálvez, and Coral Barbas   13. Assessing the energy status of low numbers of mammalian cells by capillary electrophoresis-mass spectrometry Wei Zhang, Rawi Ramautar   14. Applications of Capillary Electrophoresis-Mass Spectrometry to Chiral Analysis Elena Sánchez-López and María Luisa Marina   15. Improved Sensitivity to Determine Antibiotic Residues in Chicken Meat by In-Line Solid-Phase Extraction coupled to Capillary Electrophoresis-Tandem Mass Spectrometry Francisco J. Lara and A.M. García-Campaña   16. CE Coupled to ICP-MS and Single Particle ICP-MS for Nanoparticle Analysis Darya Mozhayeva and Carsten Engelhard  

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