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Author:   Hans J. Vogel
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2002 ed.
Volume:   173
Dimensions:   Width: 15.50cm , Height: 2.80cm , Length: 23.50cm
Weight:   1.780kg
ISBN:  

9780896036895

ISBN 10:   0896036898
Pages:   415
Publication Date:   24 January 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Methods and Techniques to Study.- Quantitative Analysis of Ca2+-Binding by Flow Dialysis.- Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators.- Deconvolution of Calcium-Binding Curves.- Absorption and Circular Dichroism Spectroscopy.- Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins.- Steady-State Fluorescence Spectroscopy.- Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins.- Surface Plasmon Resonance of Calcium-Binding Proteins.- Differential Scanning Calorimetry.- Isothermal Titration Calorimetry.- Multiangle Laser Light Scattering and Sedimentation Equilibrium.- Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in their Interactions with Regulatory Targets.- Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry.- Synthetic Calcium-Binding Peptides.- Proteolytic Fragments of Calcium-Binding Proteins.- Electron Magnetic Resonance Studies of Calcium-Binding Proteins.- Cadmium-113 and Lead-207 NMR Spectroscopic Studies of Calcium-Binding Proteins.- Calcium-43 NMR of Calcium-Binding Proteins.- Exploring Familial Relationships Using Multiple Sequence Alignment.- Structure Determination by NMR.- Protein Structure Calculation from NMR Data.- Shape and Dynamics of a Calcium-Binding Protein Investigated by Nitrogen-15 NMR Relaxation.- The Use of Dipolar Couplings for the Structure Refinement of a Pair of Calcium-Binding EGF Domains.- Vector Geometry Mapping.- Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography.- Enzymatic Assays to Compare Calmodulin Isoforms, Mutants, and Chimeras.- Gene Expression inTransfected Cells.- Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins.- Studying the Spatial Distribution of Ca2+-Binding Proteins.

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