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Author:   John N. Abelson (California Institute of Technology, Division of Biology, Pasadena, U.S.A.) ,  Melvin I. Simon (The Salk Institute, La Jolla, CA, USA) ,  John R. Sokatch (University of Texas, Austin, U.S.A.) ,  Robert Adron Harris (University of Texas, Austin, U.S.A.)
Publisher:   Elsevier Science Publishing Co Inc
Imprint:   Academic Press Inc
Volume:   324
Dimensions:   Width: 15.20cm , Height: 3.60cm , Length: 22.90cm
Weight:   0.920kg
ISBN:  

9780121822255

ISBN 10:   0121822257
Pages:   550
Publication Date:   06 September 2000
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Section I: Preparation of Substrates, Assays of Intermediates and Enzymes, and Use of Enzyme Inhibitors [1]: Synthesis and Gas Chromatography/Mass Spectrometry Analysis of Stereoisomers of 2-Hydroxy-3-methylpentanoic Acid [2]: Analysis of Intracellular Metabolites as Tool for Studying Branched-Chain Amino Acid Biosynthesis and Its Inhibition in Bacteria [3]: Determination of Branched-Chain L-Amino-Acid Aminotransferase Activity [4]: Analysis of (S)- and (R)-3-Methyl-2-oxopentanoate Enantiomorphs in Body Fluids [5]: Spectrophotometric Assay for Measuring Branched-Chain Amino Acids [6]: Determination of Branched-Chain a-Keto Acid Dehydrogenase Activity State and Branched-Chain a-Keto Acid Dehydrogenase Kinase Activity and Protein in Mammalian Tissues [7]: Simultaneous Quantification of Plasma Levels of a-Ketoisocaproate and Leucine by Gas Chromatography–Mass Spectrometry [8]: Synthesis of Methacrylyl-CoA and (R)- and (S)-3-Hydroxyisobutyryl-CoA [9]: Pathways of Leucine and Valine Catabolism in Yeast Section II: Cloning, Expression, and Purification of Enzymes of Branched-Chain Amino Acid Metabolism [10]: Isolation of Subunits of Acetohydroxy Acid Synthase Isozyme III and Reconstitution of Holoenzyme [11]: Branched-Chain Amino-Acid Aminotransferase of Escherichia coli [12]: Purification of Sodium-Coupled Branched-Chain Amino Acid Carrier of Pseudomonas aeruginosa [13]: Reconstitution of Pseudomonas aeruginosa High-Affinity Branched-Chain Amino Acid Transport System [14]: Purification of Pseudomonas putida Branched-Chain Keto Acid Dehydrogenase E1 Component [15]: Pseudomonas mevalonii 3-Hydroxy-3-methylglutaryl-CoA Lyase [16]: Human 3-Hydroxy-3-methylglutaryl-CoA Lyase [17]: Branched-Chain a-Keto Acid Dehydrogenase Kinase [18]: Expression of E1 Component of Human Branched-Chain a-Keto Acid Dehydrogenase Complex in Escherichia coli by Cotransformation with Chaperonins GroEL GroES [19]: Production of Recombinant Mammalian Holo-E2 and E3 and Reconstitution of Functional Branched-Chain a-Keto Acid Dehydrogenase Complex with Recombinant E1 [20]: Production of Recombinant E1 Component of Branched-Chain a-Keto Acid Dehydrogenase Complex [21]: Mammalian Methylmalonate-Semialdehyde Dehydrogenase [22]: Mammalian 3-Hydroxyisobutyrate Dehydrogenase [23]: 3-Hydroxyisobutyryl-CoA Hydrolase [24]: Mammalian Branched-Chain Acyl-CoA Dehydrogenases: Molecular Cloning and Characterization of Recombinant Enzymes [25]: 3-Hydroxy-3-methylglutaryl-CoA Reductase [26]: Characterization of 3-Methylcrotonyl-CoA Carboxylase from Plants [27]: Purification of D-Hydroxyisovalerate Dehydrogenase from Fusarium sambucinum [28]: Purification and Characterization of Recombinant 3-Isopropylmalate Dehydrogenases from Thermus thermophilus Other Microorganisms [29]: Wild-Type and Hexahistidine-Tagged Derivatives of Leucine-Responsive Regulatory Protein from Escherichia coli [30]: Purification of Branched-Chain Keto Acid Dehydrogenase Regulator from Pseudomonas putida [31]: Mitochondrial Import of Mammalian Branched-Chain a-Keto Acid Dehydrogenase Complex Subunits [32]: Cloning, Expression, and Purification of Mammalian 4-Hydroxyphenylpyruvate Dioxygenase/a-Ketoisocaproate Dioxygenase [33]: Mammalian Branched-Chain Aminotransferases [34]: Branched-Chain-Amino-Acid Transaminases of Yeast Saccharomyces cerevisiae [35]: Purification, Properties, and Sequencing of Aminoisobutyrate Aminotransferases from Rat Liver [36]: Branched-Chain Keto Acid Dehydrogenase of Yeast [37]: ß-Alanine Synthase an Enzyme Involved in Catabolism of Uracil and Thymine Section III: Detection and Consequences of Genetic Defects in Genes Encoding Enzymes of Branched-Chain Amino Acid Metabolism [38]: Diagnosis and Mutational Analysis of Maple Syrup Urine Disease Using Cell Cultures [39]: Detection of Gene Defects in Branched-Chain Amino Acid Metabolism by Tandem Mass Spectrometry of Carnitine Esters Produced by Cultured Fibroblasts [40]: Molecular and Enzymatic Methods for Detection of Genetic Defects in Distal Pathways of Branched-Chain Amino Acid Metabolism [41]: Genetic Defects in E3 Component of a-Keto Acid Dehydrogenase Complexes [42]: Targeting E3 Component of a-Keto Acid Dehydrogenase Complexes Section IV: Regulation and Expression of Enzymes of Branched-Chain Amino Acid Metabolism [43]: Regulation of Expression of Branched-Chain a-Keto Acid Dehydrogenase Subunits in Permanent Cell Lines [44]: Expression of Murine Branched-Chain a-Keto Acid Dehydrogenase Kinase [45]: Regulation of Branched-Chain a-Keto Acid Dehydrogenase Kinase Gene Expression by Glucocorticoids in Hepatoma Cells and Rat Liver Author Index Subject Index

Reviews

Praise for the Series The Methods in Enzymology series represents the gold-standard. --NEUROSCIENCE Incomparably useful. --ANALYTICAL BIOCHEMISTRY It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page. --BIO/TECHNOLOGY The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection. --CHEMISTRY IN INDUSTRY The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced. --AMERICAN SOCIETY OF MICROBIOLOGY NEWS If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work. --ENZYMOLOGIA A series that has established itself as a definitive reference for biochemists. --JOURNAL OF CHROMATOGRAPHY

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