Overview

The use of biocatalysts, employed either as isolated enzymes or whole microbial cells, offers a remarkable arsenal of highly selective transformations for state-of-the-art synthetic organic chemistry. Over the last two decades, this methodology has become an indispensable tool for asymmetric synthesis, not only at the academic level, but also on an industrial scale. This well-established textbook on biocatalysis provides a basis for undergraduate and graduate courses in modern organic chemistry, as well as a condensed introduction into this field. After a basic introduction into the use of biocatalysts—principles of stereoselective transformations, enzyme properties and kinetics—the different types of reactions are explained according to the 'reaction principle', such as hydrolysis, reduction, oxidation, C–C bond formation, etc. Special techniques, such as the use of enzymes in organic solvents, immobilization techniques and modified or artificial enzymes, are treated in a separate section. A final chapter deals with thebasic rules for the safe and practical handling of biocatalysts. In this completely revised 6th edition, emphasis has been given to an improved didactic style including colored graphics in order to facilitate a deeper understanding of the underlying principles. New developments, such as transamination, enzyme promiscuity and applications on industrial scale within the field of 'white biotechnology' are included.

Full Product Details

Author:   Kurt Faber
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Edition:   6th ed. 2011. 6th revised and corrected edition
Dimensions:   Width: 15.50cm , Height: 1.80cm , Length: 23.50cm
Weight:   0.772kg
ISBN:  

9783642173929

ISBN 10:   3642173926
Pages:   423
Publication Date:   28 July 2011
Audience:   College/higher education ,  Undergraduate ,  Postgraduate, Research & Scholarly
Replaced By:   9783319615899
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   Awaiting stock  

Table of Contents

1 Introduction and Background Information 1.1 Introduction.......................................................................................... 1.2 Common Prejudices Against Enzymes............................................... 1.3 Advantages and Disadvantages of Biocatalysts.................................. 1.3.1 Advantages of Biocatalysts........................................................ 1.3.2 Disadvantages of Biocatalysts................................................... 1.3.3 Isolated Enzymes Versus Whole Cell Systems......................... 1.4 Enzyme Properties and Nomenclature............................................... 1.4.1 Structural Biology in a Nutshell.............................. 1.4.2 Mechanistic Aspects of Enzyme Catalysis........................... 1.4.3 Classification and Nomenclature............................................ 1.4.4 Coenzymes....................................................................... 1.4.5 Enzyme Sources.............................................................. References..... ................................................................................... 2 Biocatalytic Applications 2.1 Hydrolytic Reactions.......................................................................... 2.1.1 Mechanistic and Kinetic Aspects............................................. 2.1.2 Hydrolysis of the Amide Bond................................................ 2.1.3 Ester Hydrolysis....................................................................... 2.1.3.1 Esterases and Proteases............................................. 2.1.3.2 Lipases....................................................................... 2.1.4 Hydrolysis and Formation of Phosphate Esters...................... 2.1.5 Hydrolysis of Epoxides........................................................... 2.1.6 Hydrolysis of Nitriles.............................................................. References........................................................................................ 2.2 Reduction Reactions..........................................................................2.2.1 Recycling of Cofactors............................................................ 2.2.2 Reduction of Aldehydes and Ketones Using Isolated Enzymes................................................................... 2.2.3 Reduction of Aldehydes and Ketones Using Whole Cells.... 2.2.4 Reduction of C=C-Bonds ....................... References........................................................................................ 2.3 Oxidation Reactions.......................................................................... 2.3.1 Oxidation of Alcohols and Aldehydes................................... 2.3.2 Oxygenation Reactions........................................................... 2.3.2.1 Hydroxylation of Alkanes........................................ 2.3.2.2 Hydroxylation of Aromatic Compounds................. 2.3.2.3 Epoxidation of Alkenes............................................ 2.3.2.4 Sulfoxidation Reactions............................................ 2.3.2.5 Baeyer-Villiger Reactions......................................... 2.3.2.6 Formation of Peroxides............................................. 2.3.2.7 Dihydroxylation of Aromatic Compounds............... 2.3.3 Peroxidation Reactions............................................................ References.... ................................................................................... 2.4 Formation of Carbon-Carbon Bonds................................................. 2.4.1 Aldol Reactions....................................................................... 2.4.2 Thiamine-Dependent Acyloin- and Benzoin Reactions......... 2.4.3 Michael-Type Additions......................................................... References........................................................................................ 2.5 Addition and Elimination Reactions................................................. 2.5.1 Cyanohydrin Formation.......................................................... 2.5.2 Addition of Water........................................... 2.5.3 Addition of Ammonia........................................... References........................................................................................ 2.6 Transfer Reactions............................................................. 2.6.1 Glycosyl Transfer Transferases.............................................. 2.6.1.1 Glycosyl Transferases........................................ 2.6.1.2 Glycosidases........................................ 2.6.2 Amino Transfer Reactions................................................... References........................................................................................ 2.7 Halogenation and Dehalogenation Reactions................................... 2.7.1 Halogenation........................................................................... 2.7.2 Dehalogenation.......................................................................References....................................................................................... 3 Special Techniques 3.1 Enzymes in Organic Solvents.......................................................... 3.1.1 Ester Synthesis........................................................................ 3.1.2 Lactone Synthesis................................................................... 3.1.3 Amide Synthesis..................................................................... 3.1.4 Peptide Synthesis.................................................................... 3.1.5 Peracid Synthesis.................................................................... 3.1.6 Redox Reactions..................................................................... 3.1.7 Medium Engineering............................................................... 3.2 Immobilization.................................................................................. 3.3 Artificial and Modified Enzymes..................................................... 3.3.1 Artificial Enzyme Mimics..................................................... 3.3.2 Modified Enzymes.......................................................... 3.3.2.1 Chemically Modified Enzymes................................ 3.3.2.2 Genetically Modified Enzymes................................ 3.3.3 Catalytic Antibodies................................................................ References........................................................................................ 4 State of the Art and Outlook................................................................. 5 Appendix 5.1 Basic Rules for Handling Biocatalysts.............................................. 5.2 Abbreviations.................................................................................... 5.3 Suppliers of Enzymes....................................................................... 5.4 Commonly Used Enzyme Preparations........................................... 5.5 Major Culture Collections................................................................ 5.6 Pathogenic Bacteria and Fungi........................................................

Reviews

Author Information

Kurt Faber obtained his PhD in synthetic organic chemistry at the University of Graz. He then became a postdoctoral fellow at the Memorial University of Newfoundland. After working as University assistant at the Institute of Organic Chemistry, University of Technology in Graz Faber was visiting senior scientist at the University of Tokyo for two years. In 1990 he became visiting senior scientist at Exeter University. In 1990 he received his habilitation in Organic Chemistry at the Institute of Organic Chemistry, University of Technology Graz where he was appointed to Associate Professor in 1997. Faber's career was supplemented again by becoming a visiting senior scientist, this time at the University of Trondheim. In 1998 he moved to the Institute of Organic Chemistry at the University of Graz where he is holding a full professorship. In 2001 he was visiting senior scientist at Stockholm University and in 2005 at the University of Minnesota.

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions