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OverviewThis is a manual which aims to give students a chance to explore the process and techniques of characterizing and purifying a protein, and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment. To minimize cost and avoid extensive laboratory preparation, the experiments in this manual use readily available materials and organisms, the bacterium Escherichia coli and the yeast Saccharomyces carlsbergensis. Laboratory topics include: microbiological methods (handling, storage, media); organism growth and protein production; enzyme and protein essays (specific activity); batch purification of proteins; gel filtration and ion exchange chromatographies; genomic and plasmid DNA isolation; and DNA sequencing and computer analysis. Full Product DetailsAuthor: David W. Burden , Donald B. WhitneyPublisher: Birkhauser Boston Inc Imprint: Birkhauser Boston Inc Edition: 1995 ed. Dimensions: Width: 17.80cm , Height: 2.10cm , Length: 23.50cm Weight: 0.812kg ISBN: 9780817637569ISBN 10: 0817637567 Pages: 336 Publication Date: 01 November 1995 Audience: College/higher education , Undergraduate , Postgraduate, Research & Scholarly Format: Hardback Publisher's Status: Out of Print Availability: Out of stock Table of ContentsReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |