Biotechnology: Proteins to PCR - A Course in Strategies and Lab Techniques

Author:   David Burden ,  Donald Whitney
Publisher:   Birkhauser Verlag AG
ISBN:  

9783764337568


Pages:   336
Publication Date:   September 1995
Replaced By:   9780817637569
Format:   Hardback
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

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Biotechnology: Proteins to PCR - A Course in Strategies and Lab Techniques


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Overview

This is a manual which aims to give students a chance to explore the process and techniques of characterizing and purifying a protein, and of subsequent cloning of the associated gene. Each chapter emphasizes the process of discovery, and the strategies and rationale for each experiment. To minimize cost and avoid extensive laboratory preparation, the experiments in this manual use readily available materials and organisms, the bacterium Escherichia coli and the yeast Saccharomyces carlsbergensis. Laboratory topics include: microbiological methods (handling, storage, media); organism growth and protein production; enzyme and protein essays (specific activity); batch purification of proteins; gel filtration and ion exchange chromatographies; genomic and plasmid DNA isolation; and DNA sequencing and computer analysis.

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Author:   David Burden ,  Donald Whitney
Publisher:   Birkhauser Verlag AG
Imprint:   Birkhauser Verlag AG
ISBN:  

9783764337568


ISBN 10:   3764337567
Pages:   336
Publication Date:   September 1995
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Replaced By:   9780817637569
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

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