Overview

This volume provides methods on procedures for assessing the biosafety aspects of probiotics. Chapters are divided into five parts detailing in vitro biosafety assessment, biogenic amine production, D-lactic acid production, toxin production, production of various enzymes, determination of toxicity, mutagenicity, virulence genes, capsule formation, hemolytic activity, DNAse activity, bile salt deconjugation, antibiotic resistance, antibiotic resistance gene transfer, mucin degradation, platelet aggregation, and in vivo biosafety assessment of probiotics including determination of  infectivity, reproductive and developmental toxicity, and evaluation of immunological parameters in animal models. Authoritative and cutting-edge, Biosafety Assessment of Probiotic Potential aims to be a foundation for future studies and to be a source of inspiration for new investigations in the field.  

Full Product Details

Author:   Mitesh Kumar Dwivedi ,  Natarajan Amaresan ,  A. Sankaranarayanan ,  Rasheedunnisa Begum
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Weight:   1.005kg
ISBN:  

9781071625088

ISBN 10:   107162508
Pages:   412
Publication Date:   25 August 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: In vitro Biosafety Assessment of Probiotics 1. Determination of biogenic Amine Production Shilpika Pandey, Archana Chaudhari, and Mitesh Kumar Dwivedi   2. Determination of Gelatinases,Glycosidases, and Enolase production Archana Chaudhari, Shilpika Pandey, and Mitesh Kumar Dwivedi   3. Determination of β-Glucuronidase Production Dixita Panchal, Vrutika Lad, Meonis Pithawala, and Natarajan Amaresan   4. Determination of Nitroreductase Production Dixita Panchal, Vrutika Lad, Meonis Pithawala, and Natarajan Amaresan   5. Determination of Azoreductase production Vrutika Lad, Dixita Panchal, Meonis Pithawala, and Natarajan Amaresan   6. Determination of Hemolytic activity Vrutika Lad, Dixita Panchal, Meonis Pithawala, Mitesh Kumar Dwivedi, and Natarajan Amaresan   7. Determination of DNAse Activity Archana Chaudhari, Ruma Raghuvanshi, and Mitesh Kumar Dwivedi   8. Determination of Bile Salts Deconjugation Hemant Borase, Satish Patil2, Mitesh Kumar Dwivedi, and Ramar Krishnamurthy   9. Determination of D-lactic Acid Production Shilpika Pandey, Archana Chaudhari, Mitesh Kumar Dwivedi   10. Determination of Antibiotic Resistance M. Veerapagu, K.R. Jeya, Ashraf Khalifa, and A. Sankaranarayanan   11. Determination of Antibiotic Resistance Gene Transfer K.R. Jeya, Ashraf Khalifa, M. Veerapagu, and A. Sankaranarayanan   12. Determination of Toxin Production Urjita V. Sheth   13. Detection of Toxin Genes by PCR Based Methods Urjita V. Sheth   14. Determination of Presence of Plasmids and Virulence and Structural Genes Teresa Semedo-Lemsaddek and Maria João Fraqueza   15. Determination of Toxicity through Cytotoxicity Assays Urjita V. Sheth   16. In vitro Evaluation of the Nitric Oxide Pathway Hemant Borase, Satish Patil, Dhruti Amin, and Mitesh Kumar Dwivedi   17. Assessment of Capsule Formation Rushabh Shah, Natarajan Amaresan, and Mitesh Kumar Dwivedi   18. Assessment of Platelet aggregation Karthick Raja Arulprakasam and Dhanasekaran Dharumadurai   19. Assessment of Fibrinogen and Fibronectin Binding Activity Suresh Singh Yadav and Pramod Kumar   20. Assessment of Probiotics Adhesion to Mammalian Cells Guhanraj Radhamanalan and Dhanasekaran Dharumadurai   21. Assessment of Mutagenicity K.R. Jeya, Ashraf Khalifa,  M. Veerapagu, and A. Sankaranarayanan   22. Assessment of Induction and Destruction of Thrombi M. Veerapagu, K.R. Jeya, and A. Sankaranarayanan   23. Assessment of degradation of mucin Ruma Raghuvanshi, Archana Chaudhari, Mitesh Kumar Dwivedi   Part II: In vivo Biosafety Assessment of Probiotics   24. Evaluation of General Health Status of the Animals during the in-Life Phase Hafiz Arbab Sakandar, Qaiser Farid Khan, Zainab Tahir, Hamid Majeed, Nabaa Azhar, and Zaib un Nisa   25. Assessment of Bacterial Translocation Through Blood Cultures Li Hui Ang, Premmala Rangasamy, Wallace Jeng Yang Chee, Shu Yih Chew, and Leslie Thian Lung Than   26. Determination of Splenic weight index and Weight-to-length ratio Firdosh Shah and Mitesh Kumar Dwivedi   27. Determination of Total liver glutathione and plasma malondialdehyde concentrations Firdosh Shah and Mitesh Kumar Dwivedi   28. Determination of Serum Lactate and Fecal Calprotectin for Assessing The Intestinal Inflammation Firdosh Shah and Mitesh Kumar Dwivedi   Part III: Measuring infectivity in animal models   29. In vivo Evaluation of Adhesion properties of Probiotics Nabendu Debnath, Pooja Yadav, Ashish Tyagi, and Ashok Kumar Yadav   30. Determination of Streptomyces Probiotics oral administration in broiler chicken Latha Selvanathan, Thirumagal Thirugnanam, Vinothini Gopal and Dhanasekaran Dharumadurai   31. Determination of infectivity of probiotics using animal models Shabari Girish and Lokesh Ravi   32. Determination of Infectivity of Probiotics using Immunosuppressed Hosts Sumana Chatterjee and Tamalika Chakraborty   Part IV: Measuring Reproductive and Developmental toxicity in animal models 33. Assessment of Reproductive toxicity Firdosh Shah and Mitesh Kumar Dwivedi   34. Assessment of Developmental toxicity in Zebrafish model Tamalika Chakraborty, Sumana Roy, Dipanjan Mandal, and Jeenatara Begum   Part V: Measuring Immunological Parameters in animal models for Safety   35. Assessment of Inflammation in Animal Models (Macroscopic Or Histological Inflammation In The Ileum Or In The Colon)  Sumana Roy, Tamalika Chakraborty, Prerona Saha, and Sriparna Kundu Sen   36. Assessment of inflammation in animal models (Quantification of TNFA, IFNG, IL4, and IL10 mRNAs by Real-time PCR) Sachin Kumar, Rashmi H.M., and Brijesh Kumar   37. Assessment of inflammation in animal models (Quantification of TNF-α, IFN-γ, IL-4, & IL-10 proteins by ELISA) Siti Nor Hikmah Abdul Rasid, Angela Boahen, Shu Yih Chew, Shalini Vellasamy, and Leslie Thian Lung Than   38.  Detection of Myeloperoxidase activity by Enzyme Linked Immunosorbent Assay Pooja Yadav, Nabendu Debnath, Ashish Tyagi, and Ashok Kumar Yadav   39. Assessment of Bacterial Translocation Through Mesenteric Lymph Nodes [MLN] and Spleen Cultures Fida Fathima and Rekha P.D.

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions