Bioluminescent Imaging: Methods and Protocols

Author:   Christian E. Badr
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2014
Volume:   1098
ISBN:  

9781493960675


Pages:   276
Publication Date:   23 August 2016
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Bioluminescent Imaging: Methods and Protocols


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Full Product Details

Author:   Christian E. Badr
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2014
Volume:   1098
Dimensions:   Width: 17.80cm , Height: 1.50cm , Length: 25.40cm
Weight:   5.453kg
ISBN:  

9781493960675


ISBN 10:   1493960679
Pages:   276
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Bioluminescence Imaging: Basics and Practical Limitations.- Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells.- Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter.- Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture.- Split Gaussia Luciferase for Imaging Ligand-Receptor Binding.- Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell.- Bioluminescence Reporter Gene-Based Detection of MicroRNAs.- Monitoring of Transcriptional Dynamics of HIF and NFκB Activities.- Real-Time Bioluminescent Tracking of Cellular Population Dynamics.- Fabrication of Bioluminescent Capsules and Live-Cell Imaging.- The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice.- Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis.- Bioluminescence Imaging of Fungal Biofilm Development in Live Animals.- Bioluminescent Imaging of Bacteria During Mouse Infection.- Cell-Based Bioluminescence Screening Assays.- Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice.- Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase.- Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay.- Non-Invasive In Vivo Monitoring of Extracellular Vesicles.- In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL).

Reviews

From the reviews: The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. ... The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields. (Omer Iqbal, Doody's Book Reviews, January, 2014)


From the reviews: The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. ... The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields. (Omer Iqbal, Doody's Book Reviews, January, 2014)


From the reviews: “The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. … The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields.” (Omer Iqbal, Doody's Book Reviews, January, 2014)


From the reviews: The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. ... The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields. (Omer Iqbal, Doody's Book Reviews, January, 2014)


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