Overview

Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. *A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab*Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates*More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates*Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs, fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions

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9781281370907

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This is a well-organized and well-written book; it is readable and comprehensible to the novice, but its content and stylistic approach seem sufficiently sophisticated to appeal to active, knowledgeable workers in the field. It is an impressive compilation of useful theoretical and practical information that is not readily available elsewhere in a single volume. --Joseph G. Cannon in JOURNAL OF MEDICINAL CHEMISTRY The presentation throughout is that of a laboratory manual. Well-illustrated descriptions of a particular chemical reaction are followed by a detailed protocol and list of references extending into 1994. The coverage of reagents and methods is comprehensive, and sources are given for commercially available compounds. --NATURE

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