Overview

"The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make ""Microbiological Applications: A Laboratory Manual in General Microbiology"" the ideal lab manual. Appropriate for either a majors or non-majors lab course, ""Benson"" assumes no prior organic chemistry course has been taken."

Full Product Details

Author:   Alfred E. Brown
Publisher:   McGraw-Hill Education - Europe
Imprint:   McGraw Hill Higher Education
Edition:   9th Revised edition
Dimensions:   Width: 22.10cm , Height: 1.90cm , Length: 27.10cm
Weight:   1.043kg
ISBN:  

9780072823981

ISBN 10:   0072823984
Pages:   528
Publication Date:   04 March 2004
Audience:   College/higher education ,  Tertiary & Higher Education
Format:   Spiral bound
Publisher's Status:   Active
Availability:   Out of stock  
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Laboratory ProtocolSafety 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Fluorescence Microscopy5 Microscopic Measurements2 Survey of Microorganisms6 Protozoans, Algae, and Cyanobacteria7 Ubiquity of Bacteria8 The Fungi: Yeasts and Molds3 Manipulation of Microorganisms9 Aseptic Technique10 Pure Culture Techniques4 Staining and Observation of Microorganisms11 Smear Preparation12 Simple Staining13 Negative Staining14 Capsular Staining15 Gram Staining16 Spore Staining: Two Methods17 Acid-Fast Staining: Ziehl-Neelsen Method18 Motility Determination5 Culture Methods19 Culture Media Preparation20 Preparation of Stock Cultures21 Cultivation of Anaerobes22 Enumeration of Bacteria: The Standard Plate Count23 Slime Mold Culture24 Slide Culture: Molds6 Bacterial Viruses25 Determination of a Bacteriophage Titer26 Burst Size Determination: A One-Step Growth Curve 27 Isolation of Phage from Flies28 Phage Typing7 Environmental Influences and Control of Microbial Growth29 Temperature: Effects on Growth30 Temperature: Lethal Effects31 pH and Microbial Growth32 Water Activity and Osmotic Pressure33 Ultraviolet Light: Lethal Effects34 Oligodynamic Action35 Evaluation of Alcohol: Its Effectiveness as an Antiseptic36 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method37 Evaluation of Antiseptics: The Filter Paper Disk Method38 Effectiveness of Hand Scrubbing8 Identification of Unknown Bacteria39 Morphological Study of Unknown Bacterium40 Cultural Characteristics41 Physiological Characteristics: Oxidation and Fermentation Tests42 Physiological Characteristics: Hydrolytic Reactions43 Physiological Characteristics: Biochemical Tests44 Use of Bergey's Manual9 Miniaturized Multitest Systems45 Enterobacteriaceae Identification: The API 20E System46 Enterobacteriaceae Identification: The Enterotube II System 47 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System48 Staphylococcus Identification: The API Staph-Ident System10 Diversity and Environmental Microbiology49 Isolation of an Antibiotic Producer: The Actinomyces50 The Nitrogen Cycle: Amonification51 Symbiotic Nitrogen Fixation: Rhizobium52 Free-living Nitrogen Fixation: Azotobacter53 Denitrifcation: Paracoccus denitrificans54 The Winogradsky Column55 Purple Nonsulphur Photosynthetic Bacteria56 Sulfate Reducing Bacteria: Desulfovibrio57 Bacterial Commensalism58 Bacterial Synergism59 Microbial Antagonism11 Applied Microbiology60 Bacterial Counts of Foods61 Bacterial Examination of Water: Qualitative Tests62 The Membrane Filter Method63 Reductase Test64 Microbial Spoilage of Canned Food65 Microbiology of Alcohol Fermentation12 Bacterial Genetics and Biotechnology66 Mutant Isolation by Replica Plating67 Bacterial Transformation 68 Polymerase Chain Reaction for Amplifying DNA69 Plasmid Isolation13 Medical Microbiology and Immunology70 The Staphylococci: Isolation and Identification71 The Streptococci: Isolation and Identification72 Gram Negative Intestinal Pathogens73 Slide Agglutination Test: Serological Typing74 Slide Agglutination (Latex) Test: For S. aureus Identification75 Tube Agglutination Test: The Heterophile Antibody Test76 White Blood Cell Study: The Differential WBC Count77 Blood Grouping78 A Synthetic Epidemic AppendixesA TablesB Indicators, Stains, Reagents C MediaD Identification ChartsE The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics

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Professor and Chair, Consultant Environmental Microbiology

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