Overview
This second edition provides updated and new chapters to build on and extend the strengths of the first edition. Chapters guide readers through basic biology of basophils, obtaining the cells by purification, culture of stem cells progenitors, peripheral CD34+ stem cell-derived mast cells, basophils from CD34+ progenitors, diagnostic applications, gene expression patterns in basophils, roles of basophils in different asthma phenotypes, knockout, and disease models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Basophils and Mast Cells: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Full Product Details
Author: Bernhard F. Gibbs , Franco H. Falcone
Publisher: Springer-Verlag New York Inc.
Imprint: Springer-Verlag New York Inc.
Edition: 2nd ed. 2020
Volume: 2163
Weight: 0.758kg
ISBN: 9781071606988
ISBN 10: 1071606980
Pages: 380
Publication Date: 08 August 2021
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Paperback
Publisher's Status: Active
Availability: Manufactured on demand 
We will order this item for you from a manufactured on demand supplier.
Table of Contents
Part I: Introduction and Historical Background 1. Paradigm Shifts in Mast Cell and Basophil Biology and Function: An Emerging View of Immune Regulation in Health and Disease Ana Olivera and Juan Rivera Part II: Obtaining the Cells: Purification, Culture, and Counting 2. Purification of Basophils from Peripheral Human Blood Franco H. Falcone and Bernhard F. Gibbs 3. Mast Cell Purification Protocols Jasmine Farrington, Elizabeth P Seward, and Peter T Peachell 4. Generation of a Human Allergic Mast Cell Phenotype from CD133+ Stem Cells Hans Jürgen Hoffmann 5. Generation and Culture of Peripheral CD34+ Stem Cell-derived Mast Cells (PSCMCs) Yanyan Luo, Stefan Frischbutter, Jörg Scheffel, and Frank Siebenhaar 6. Modulating the Human Basophil Phenotype During its Development and Maturation: Basophils Derived from in vitro Cultures of CD34+ Progenitor Cells Donald MacGlashan Jr. 7. Generation of Mast Cells from Murine Stem Cell Progenitors Emily J. Swindle 8. Integration of the Human Dermal Mast Cell into the Organotypic Co-culture Skin Model Jonghui Kim, Sven Guhl, Magda Babina, Torsten Zuberbier, and Metin Artuc 9. The Absolute Basophil Count Elena Borzova Part III: Basophil/Mast Cell Lines 10. Mast Cell and Basophils Cell Lines: A Compendium Egle Passante 11. Use of Humanized RBL Reporter Systems for Detection of Allergen-specific IgE Sensitization in Human Serum Daniel Wan, Xiaowei Wang, Ryosuke Nakamura, Marcos J.C. Alcocer, and Franco H. Falcone 12. Use of Humanized Fluorescent Reporter Cell Line RBL NFAT-DsRed for Detection of Allergen-specific IgE in Patient Sera Using Allergen Microarrays Marina Kalli, Marcos J. C. Alcocer, Andrew J. Blok, and Franco H. Falcone 13. NPY-mRFP Rat Basophil Leukemia (RBL) Reporter: A Novel, Fast Reporter of Basophil/Mast Cell Degranulation Nafal Barwary, Daniel Wan, and Franco H. Falcone 14. Use of Engineered Nanoparticles (ENPs) for the Study of High-affinity IgE Receptor FcεRI Receptor Engagement and Rat Basophil Leukemia (RBL) Cell degranulation Alelwani W, Alharbi RA , Wan D , Vllasaliu D, Falcone FH , and Stolnik S Part IV: Diagnostic Applications 15. Flow Cytometric Allergy Diagnosis: Basophil Activation Techniques Chris H. Bridts, Vito Sabato, Christel Mertens, Margo M. Hagendorens, Luc S. De Clerck, and Didier G. Ebo 16. Basophil Activation Experiments in Immediate Drug Hypersensitivity: More Than a Diagnostic Aid Didier G Ebo, Jessy Elst, Athina Van Gasse, Leander De Puysseleyr, Margaretha Faber, Margo M Hagendorens, Lina Mayorga, Christel Mertens , Chris H Bridts, Luc S De Clerck, and Vito Sabato 17. Basophil Activation Techniques: Staining of Exteriorized Granule Matrix Jessy Elst, Vito Sabato, Margo M Hagendorens, Michel van Houdt, Margaretha A. Faber, Chris H Bridts, Didier G Ebo, and Christel Mertens 18. Measurement and Functional Analysis of the Mas-related G protein-coupled receptor MRGPRX2 on Human Mast Cells and Basophils Jessy Elst, Vito Sabato, Margo M Hagendorens, Michel van Houdt, Margaretha A. Faber, Chris H Bridts, Didier G Ebo, and Christel Mertens 19. The Mast Cell Activation Test (MAT) Rajia Bahri and Silvia Bulfone-Paus Part V: Whole Cells: Functional Studies 20. Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique Victor G. Bechmann, Anette Teo Hansen, Lars K. Poulsen, and Bettina M. Jensen 21. Measuring Histamine and Cytokine Release from Basophils and Mast Cells Bettina M. Jensen, Ewa A. Bartko, Katrine Baumann, and Per S. Skov 22. Detection of Mast Cells and Basophils by Immunohistochemistry Andrew F. Walls and Cornelia Amalinei 23. Detection of Basophils and Other Granulocytes in Induced Sputum by Flow Cytometry Lionel Rohner and Michaela Fux 24. Mast Cell Migration and Chemotaxis Assayed by Microscopy Monika Bambousková, Zuzana Rubíková, Lubica Dráberová, Pavel Dráber, and Petr Dráber 25. Basophil Stimulation and Signaling Pathway Edward F. Knol and Bernhard F. Gibbs 26. Gene Silencing Approaches in Mast Cells and Primary Human Basophils Vadim V. Sumbayev and Bernhard F. Gibbs 27. Identification and Immunophenotypic Characterization of Normal and Pathological Mast Cells José Mário Morgado, Laura Sánchez-Muñoz, Cristina Teodósio, and Luís Escribano Part VI: Whole Organism: Disease and Knockout Models 28. Mast Cell Mediated Reactions in vivo Stefan Frischbutter, Jörg Scheffel, and Frank Siebenhaar 29. Identification of Murine Basophils by Flow Cytometry and Histology Christian Schwartz and David Voehringer
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