Overview

Full Product Details

Author:   John M. Walker
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1994 ed.
Volume:   32
Dimensions:   Width: 15.50cm , Height: 2.60cm , Length: 23.50cm
Weight:   1.940kg
ISBN:  

9780896032682

ISBN 10:   089603268
Pages:   490
Publication Date:   26 April 1994
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

The Lowry Method for Protein Quantitation.- The Bicinchoninic Acid (BCA) Assay for Protein Quantitation.- The Bradford Method for Protein Quantitation.- Nondenaturing Polyacrylamide Gel Electrophoresis of Proteins.- SDS Polyacrylamide Gel Electrophoresis of Proteins.- Gradient SDS Polyacrylamide Gel Electrophoresis of Proteins.- Acetic Acid-Urea Polyacrylamide Gel Electrophoresis of Proteins.- Electrophoresis of Peptides.- Isoelectric Focusing of Proteins in Ultra-Thin Polyacrylamide Gels.- The In Vivo Isotopic Labeling of Proteins for Polyacrylamide Gel Electrophoresis.- Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins.- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Immobilized pH Gradients in the First Dimension.- Two-Dimensional Polyacrylamide Gel Electrophoresis Using Flat-Bed Isoelectric Focusing in the First Dimension.- Quantification of Proteins on Polyacrylamide Gels (Nonradioactive).- Detection of Proteins in Polyacrylamide Gels Using an Ultrasensitive Silver Staining Technique.- Identification of Glycoproteins on Nitrocellulose Membranes and Gels.- The Release of Oligosaccharides from Glycoproteins.- Structural Profiling of Oligosaccharides of Glycoproteins.- Drying Gels.- Fluorography of Polyacrylamide Gels Containing Tritium.- The Electrophoretic Elution of Proteins from Polyacrylamide Gels.- Preparation of Proteins from Gels for Protein Microsequencing.- Comparison of Protein Primary Structures.- Protein Blotting.- Detection of Polypeptides on Immunoblots Using Secondary Antibodies or Protein A.- Detection of Proteins on Blots Using the Avidin-Biotin System.- Detection of Proteins on Protein Blots Using Chemiluminescent Systems.- The Detection of Proteins on Blots Using Gold or Immunogold.- Amino Acid Analysis.- Molecular-Weight Estimation for Native Proteins Using Size-Exclusion High-Performance Liquid Chromatography.- Peptide Purification by Reverse-Phase HPLC.- Enzymatic Methods for Cleaving Proteins.- Chemical Cleavage of Proteins.- Chemical Modification of Proteins.- The Dansyl Method for Identifying N-Terminal Amino Acids.- The Dansyl-Edman Method for Peptide Sequencing.- C-Terminal Sequencing of Peptides.- Analysis of Cysteine Residues and Disulfide Bonds.- Preparation and Characterization of Monoclonal Antibodies to Proteins and Other Cellular Components.- The Raising of a Polyclonal Antiserum to a Protein.- Production of Antisera to Synthetic Peptides.- Production of Antibodies Using Proteins in Gel Bands.- Preparation of Purified Immunoglobulin G (IgG).- Enzyme and Biotin Labeling of Antibody.- Labeling of Peptides and Proteins by Radioiodination.- Radioimmunoassay of Peptides and Proteins.- Enzyme-Linked Immunosorbent Assay (ELISA).- Free Zone Capillary Electrophoresis.

Reviews

...would I buy this volume? I think that the answer to this is yes. It is a useful and concise volume that would be of great value around the lab. I would also recommend it for libraries as it provides an excellent reference source on techniques of protein analysis for undergraduates entering the difficult world of research projects. The value of this volume lies in the fact that any potential reader is liable to use the techniques described in several chapters to pursue a piece of research work. - Trends in Biochemical Sciences

...would I buy this volume? I think that the answer to this is yes. It is a useful and concise volume that would be of great value around the lab. I would also recommend it for libraries as it provides an excellent reference source on techniques of protein analysis for undergraduates entering the difficult world of research projects. The value of this volume lies in the fact that any potential reader is liable to use the techniques described in several chapters to pursue a piece of research work. - Trends in Biochemical Sciences

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions