Overview
Confocal scanning laser microscopy has become a primary method in many laboratories for visualizing microscopic structure in the third and fourth dimensions. However, in many cases individual users are expected to understand and operate complex confocal microscopes without being given the opportunity to learn basic principals. This text will target beginning and intermediate users of confocal microscopy and will include information that students, technologists and researchers need to know to efficiently collect and analyze data and to obtain publication quality images. The first part of the volume will provide information on a number of topics relevant to confocal microscopy, including the basics of fluorescence and fluorescent probes, biological specimen preparation (fixation, staining, optical properties and mounting materials), the basic components of a confocal microscope (lasers, dichroic mirrors, microscope objectives, photomultiplier tubes, etc.) and an overview of some applications of confocal microscopy. Proper selection of user adjustable parameters to optimize image collection will also be addressed and demonstrated.
Full Product Details
Author: Robert L. Price ,
W. Gray (Jay) Jerome
Publisher: Springer-Verlag New York Inc.
Imprint: Springer-Verlag New York Inc.
Edition: 2011 ed.
Dimensions:
Width: 15.50cm
, Height: 1.90cm
, Length: 23.50cm
Weight: 0.637kg
ISBN: 9780387781747
ISBN 10: 0387781749
Pages: 302
Publication Date: 22 April 2011
Audience:
College/higher education
,
Professional and scholarly
,
Undergraduate
,
Postgraduate, Research & Scholarly
Replaced By: 9783319974538
Format: Hardback
Publisher's Status: Out of Print
Availability: In Print
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.
Reviews
From the reviews: This is an eleven chapter's effort done by a bunch of Authors coordinated by Prof. R.L. Price and W.G. Jerome ... that with great skills are revealing us the secrets of confocal microscopy. ... The chapters devoted to the conversion of analogic into digital data make the book particularly valuable. ... Definitely an excellent book, a must for the microscopists and anyone who is using a microscope and/or is studying biology. (Manuela Monti, European Journal of Histochemistry, Vol. 56 (1), 2012)
From the reviews: This is an eleven chapter's effort done by a bunch of Authors coordinated by Prof. R.L. Price and W.G. Jerome ... that with great skills are revealing us the secrets of confocal microscopy. ... The chapters devoted to the conversion of analogic into digital data make the book particularly valuable. ... Definitely an excellent book, a must for the microscopists and anyone who is using a microscope and/or is studying biology. (Manuela Monti, European Journal of Histochemistry, Vol. 56 (1), 2012)
From the reviews: This is an eleven chapter's effort done by a bunch of Authors coordinated by Prof. R.L. Price and W.G. Jerome ... that with great skills are revealing us the secrets of confocal microscopy. ... The chapters devoted to the conversion of analogic into digital data make the book particularly valuable. ... Definitely an excellent book, a must for the microscopists and anyone who is using a microscope and/or is studying biology. (Manuela Monti, European Journal of Histochemistry, Vol. 56 (1), 2012)
