Basic Cloning Procedures
Author:   Valdis Berzins
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Edition:   1998 ed.
ISBN:  

9783540635390


Pages:   176
Publication Date:   10 March 1998
Format:   Book
Availability:   Out of stock   Availability explained
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

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Basic Cloning Procedures


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Overview

This manual introduces the reader to basic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis. Each protocol includes short background information, a detailed description of the necessary materials, step-by-step procedures, a troubleshooting guide and useful practical hints.

Full Product Details

Author:   Valdis Berzins
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Edition:   1998 ed.
Dimensions:   Width: 15.50cm , Height: 1.40cm , Length: 23.50cm
Weight:   0.315kg
ISBN:  

9783540635390


ISBN 10:   3540635394
Pages:   176
Publication Date:   10 March 1998
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Book
Publisher's Status:   Active
Availability:   Out of stock   Availability explained
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Juris Steinbergs, Aleksander Tsimanis: cDNA Synthesis and Cloning.- Eriks Jankevics: DNA Sequencing. - Elita Avota, Normunds Licis: RNA in Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase.- Janis Klovins, Valdis Berzins: PCR-Based Site-Specific Mutagenesis.- Indrikis Muiznieks, Nils Rostoks: Analysis of Specific Protein--DNA Interactions.- Illar Pata, Erkki Truve: Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids.- Irina Sominskaya, Kaspars Tars: Immunological Methods for Analysis of Recombinant Proteins.- Tiina Tamm, Erkki Truve: Protein Synthesis in Cell-Free Systems.

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