Overview
This volume explores base editors (BEs), an invaluable CRISPR-based genome editing tool with a wide variety of versatile applications. Beginning with an overview of BEs, their diverse variants, and computational tools, the book continues with experimental applications of BEs for disease modeling in mammalian cells and generating mutagenic mice, therapeutic base editing strategies, which covers delivery methods of BE-encoded DNA plasmids, mRNAs, or ribonucleoproteins through viruses or non-viral lipid nanoparticles, and lastly, the use of BEs in plants and bacteria. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Base Editors: Methods and Protocols serves as an ideal guide for researchers looking to use base editors to continue their studies in an array of fields.
Full Product Details
Author: Sangsu Bae , Beomjong Song
Publisher: Springer-Verlag New York Inc.
Imprint: Springer-Verlag New York Inc.
Edition: 1st ed. 2023
Volume: 2606
Weight: 0.671kg
ISBN: 9781071628782
ISBN 10: 107162878
Pages: 235
Publication Date: 03 January 2023
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Hardback
Publisher's Status: Active
Availability: Manufactured on demand 
We will order this item for you from a manufactured on demand supplier.
Table of Contents
Part I: Overview of BEs, Diverse Variants of BEs, and Computational Tools for Bes 1. Introduction and Perspectives of DNA Base Editors Beomjong Song and Sangsu Bae 2. Web-Based Computational Tools for Base Editors Gue-Ho Hwang and Sangsu Bae 3. Prediction of Base Editing Efficiencies and Outcomes Using DeepABE and DeepCBE Jinman Park and Hui Kwon Kim 4. Profiling Genome-Wide Specificity of dCpf1 Cytidine Base Editors Using Digenome-Seq Daesik Kim Part II: Application of BEs for Disease Modeling 5. Base Editing of Human Hematopoietic Stem Cells Jing Zeng, Gabriele Casirati, My Anh Nguyen, Pietro Genovese, and Daniel E. Bauer 6. A/C Simultaneous Conversion Using the Dual Base Editor in Human Cells Xiaohui Zhang, Yuting Guan, and Dali Li 7. Functional Analysis of Variants in BRCA1 Using CRISPR Base-Editors Ji-Eun See and Yongsub Kim 8. Use of the Representative Base Editing Tool Target-AID to Introduce Pathogenic Mutations into Mice Hiroki Sasaguri 9. Targeted Mutagenesis in Mice Using a Base Editor Tae Yeong Jeong, Soo-Yeon Lim, Je Kyung Seong, and Kyoungmi Kim Part III: Therapeutic Base Editing Strategies 10. Heterologous Expression and Purification of a CRISPR-Cas9-Based Adenine Base Editor Seu-Na Lee, Hong-Su Jang, and Jae-Sung Woo 11. Delivering Base Editors In Vivo by Adeno-Associated Virus Vectors Colin K.W. Lim, Angelo J. Miskalis, Pablo Perez-Pinera, and Thomas Gaj 12. The Delivery of ABE mRNA to the Adult Murine Liver by Lipid Nanoparticles (LNPs) Dong Yang and Chun-Qing Song 13. Ex Vivo Base Editing Therapy with Chemically-Derived Hepatic Progenitors Yohan Kim, Jaemin Jeong, and Dongho Choi 14. Application of Base Editor-Mediated Genome Editing in Mouse Retina Jun Wu, Chang Sik Cho, Dong Hyun Jo, and Jeong Hun Kim Part IV: Use of BEs in Plants and Bacteria 15. High-Throughput Base-Editing-Mediated Artificial Evolution Streamlines Trait Gene Identification in Rice Fang Yan, Man Yu, Meixia Wang, and Huanbin Zhou 16. Heritable Virus-Induced Genome Editing (VIGE) in Nicotiana attenuate Hyeonjin Kim, Youngbin Oh, Eunae Park, Moonyoung Kang, Yuri Choi, and Sang-Gyu Kim 17. Cytosine Base Editing in Bacteria Ye Liu, Yang Liu, Ping Zheng, Yu Wang, and Meng Wang
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