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Author:   David R. O'Reilly (Department of Biology, Department of Biology, Imperial College, London) ,  Lois K. Miller (Departments of Entomology and Genetics, Departments of Entomology and Genetics, University of Georgia) ,  Verne A. Luckow (, Monsanto Corporation, Chesterfield, Maryland)
Publisher:   Oxford University Press Inc
Imprint:   Oxford University Press Inc
Edition:   New edition
Dimensions:   Width: 27.90cm , Height: 2.30cm , Length: 23.50cm
Weight:   0.774kg
ISBN:  

9780195091311

ISBN 10:   0195091310
Pages:   368
Publication Date:   12 May 1994
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Spiral bound
Publisher's Status:   Active
Availability:   To order  
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Table of Contents

Acknowledgments How To Use This Manual PART I: AN OVERVIEW OF BACULOVIRUSES 1: Virus Structure and the Infection Process 2: Gene Organization, Regulation, and Function 3: Virus-Host Interactions 4: Summary of Baculovirus Features Relevant to Expression Factors PART II: CHOOSING A TRANSFER PLASMID AND PARENT VIRUS 5: Choice of Virus and Host Species 6: Choice of Transfer Plasmid 7: Available Transfer Plasmids 8: Choosing a Parent Virus for Use in Vector Construction 9: Optimizing Expression: Tailoring the Heterologous Gene to the Transfer Plasmid and the Baculovirus Expression System PART III: METHODS FOR VECTOR CONSTRUCTION AND GENE EXPRESSION 10: Safety Considerations 11: Insect Cell Culture 12: Virus Methods 13: Contransfection and Recombinant Virus Identification 14: Characterizing Recombinant Gene Expression 15: Post-Translational Modification PART IV: METHODS FOR SCALE-UP OF PROTEIN PRODUCTION AND USE OF INSECT LARVAE 16: David L. Clemm: Scale-Up of Protein Production in a Stirred Bioreactor 17: Cheryl Isaac Murphy: Scale-Up of Protein Production in an Airlift Fermenter 18: Insect Rearing and Infection Appendix 1: Nucleotide Sequence of AcMNPV Polyhedrin Region Appendix 2: Nucleotide Sequence of AcMNPV p10 Region Appendix 3: List of Genes Expressed in the Baculovirus Expression System Appendix 4: Suppliers Appendix 5: Stock Solutions Appendix 6: Excel Spreadsheet for TCID[5[0 Calculation References Index

Reviews

A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work. --Cell<br> Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview prior to taking the plunge. --Tibtech<br>

A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work. --Cell Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview prior to taking the plunge. --Tibtech

A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work. --Cell Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview prior to taking the plunge. --Tibtech A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work. --Cell Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview prior to taking the plunge. --Tibtech A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work. --Cell Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview prior to taking the plunge. --Tibtech A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work. --Cell Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview prior to taking the plunge. --Tibtech A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this areawho wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work. --Cell Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of thequestions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of anyone using the system at present, or who requires an overview prior to taking the plunge. --Tibtech

<br> A dedicated monograph. . . . a brief but thorough and well-referenced review . . . very well written. It will prove to be equally useful to first-time investigators embarking on an adventure involving baculovirus-mediated gene expression and to those of us with considerable experience in this area who wish to have a good manual for quick reference. I would classify this book as the baculovirus equivalent of the best-selling Molecular Cloning by Sambrook, et al. and recommend it as an important primer for any lab that plans to become or is currently involved in this type of work. --Cell<br> Short and sharp. Excellent restriction maps of plasmid transfer vectors. . . . the book describes all aspects of the baculovirus expression system in sufficient detail to enable the uninitiated to achieve success. The book contains a vast amount of useful information and should answer most of the questions posed by users of the baculovirus system. . . . a valuable addition to the bookshelf of a

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