Overview

Experienced researchers describe in step-by-step detail powerful methods for the investigation of bacterial exo- and endotoxins. These state-of-the-art techniques range from purification and detection methods to methods of conformational analysis, and include the use of phage antibody libraries and methods of structural and functional analyses of endotoxins. Each protocol is described by a scientist who has regularly used the method and optimized it to a high level of performance. Extensive notes deal with the difficulties that may arise when using the method and with the method's limitations and possible modification for other purposes. Cutting-edge and geared to producing successful experimental results, Bacterial Toxins: Methods and Protocols provides investigators with a first-rate collection of readily reproducible methods designed to help today's bacterial toxin investigators - both novice and expert - utilize a wide array of powerful research tools.

Full Product Details

Author:   Otto Holst
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2000 ed.
Volume:   145
Dimensions:   Width: 15.50cm , Height: 2.20cm , Length: 23.50cm
Weight:   1.610kg
ISBN:  

9780896036048

ISBN 10:   0896036049
Pages:   379
Publication Date:   10 March 2000
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of print, replaced by POD  
We will order this item for you from a manufatured on demand supplier.

Table of Contents

Bacterial Protein Toxins.- Purification of Clostridium botulinum Type A Neurotoxin.- Shiga Toxins.- Isolation and Detection of Microcystins and Nodularins, Cyanobacterial Peptide Hepatotoxins.- Genetic Construction, Expression, and Characterization of Diphtheria Toxin-Based Growth Factor Fusion Proteins.- Structure-Function Analysis of Cysteine-Engineered Entomopathogenic Toxins.- Use of Fourier-Transformed Infrared Spectroscopy for Secondary Structure Determination of Staphylococcal Pore-Forming Toxins.- The Use of Fluorescence Resonance Energy Transfer to Detect Conformational Changes in Protein Toxins.- Site-Directed Spin Labeling of Proteins.- Characterization of Molecular Properties of Pore-Forming Toxins with Planar Lipid Bilayers.- Determination of Affinity and Kinetic Rate Constants Using Surface Plasmon Resonance.- ADP-Ribosylation of ?-Gi Proteins by Pertussis Toxin.- Phage Libraries for Generation of Anti-Botulinum scFv Antibodies.- T-Cell Cytotoxicity Assays for Studying the Functional Interaction Between the Superantigen Staphylococcal Enterotoxin A and T-Cell Receptors.- In Vitro Physiological Studies on Clostridial Neurotoxins.- The Biology of Endotoxin.- Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of Lipopolysaccharides.- Applications of Combined Capillary Electrophoresis-Electrospray Mass Spectrometry in the Characterization of Short-Chain Lipopolysaccharides.- Deacylation of Lipopolysaccharides and Isolation of Oligosaccharide Phosphates.- Electrophysiological Measurements on Reconstituted Outer Membranes.

Reviews

The editor has put together an outstanding text on the current protocols used in the study of protein toxins. The caliber of the contributors is outstanding and represents an international perspective. While the editor does not present the text as an exhaustive discussion of microbial toxins, there is a focus on those areas where there is current and active research such as toxin-receptor kinetics, the use of fluorescence resonance energy transfer to detect structural changes in protein toxins, and CE-ESMS of short-chain lipopolysaccharides in Haemophilus influenzae. The organization and content level of this manual makes it a highly desirable addition to either a personal or a departmental library. -Doody's Health Science Book Review Journal

The editor has put together an outstanding text on the current protocols used in the study of protein toxins. The caliber of the contributors is outstanding and represents an international perspective. While the editor does not present the text as an exhaustive discussion of microbial toxins, there is a focus on those areas where there is current and active research such as toxin-receptor kinetics, the use of fluorescence resonance energy transfer to detect structural changes in protein toxins, and CE-ESMS of short-chain lipopolysaccharides in Haemophilus influenzae. The organization and content level of this manual makes it a highly desirable addition to either a personal or a departmental library. - Doody's Health Science Book Review Journal

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